Chimeric casein and whey proteins and use thereof
AssigneeImagindairy Ltd.
InventorsArie ABO, Ariella LAVY-ALPEROVITCH
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Abstract
The present invention provides a chimeric polynucleotide comprising: a first nucleic acid sequence encoding a first polypeptide selected from: alpha S1 casein, alpha S2 casein, and beta casein, a whey polypeptide, or a functional analog thereof, a second nucleic acid sequence encoding a cleavage site of a proteolytic enzyme; and, a third nucleic acid sequence encoding a kappa casein macropeptide, Further provided are a chimeric polypeptide, as well as compositions comprising same, and methods of using same, such as for producing an edible composition comprising casein and/or whey polypeptide aggregates, such as a dairy substituent curd.
Claims22
A chimeric polynucleotide comprising:
- a) a first nucleic acid sequence encoding a first polypeptide selected from the group consisting of:
- alpha S1 casein, alpha S2 casein, beta casein, whey polypeptide, and any functional analog thereof;
- b) a second nucleic acid sequence encoding a cleavage site of a proteolytic enzyme;
- and
- c) a third nucleic acid sequence encoding a kappa casein macropeptide. wherein said second nucleic acid sequence is positioned between said first and third nucleic acid sequences, and wherein said first, second, and third nucleic acid sequences are operably linked.
(canceled)
A method for producing an edible composition comprising casein and/or whey polypeptide aggregates, the method comprising providing a composition comprising a chimeric polypeptide comprising:
- (i) a first polypeptide being a polypeptide selected from the group consisting of:
- alpha S1 casein, alpha S2 casein, beta casein, whey polypeptide, whey polypeptide, and a functional analog thereof;
- and (ii) a second polypeptide being a kappa casein macropeptide, wherein said first polypeptide and said second polypeptide are interspaced by a cleavage site of a proteolytic enzyme, and contacting said composition with an effective amount of said proteolytic enzyme, thereby producing the edible composition comprising casein and/or whey polypeptide aggregates.
-22. (canceled)
Dependencies are inferred from the claim text.