Manufacturing systems and methods for cellular therapeutic platforms
OwnerRemo MOOMIAIE, Richard KLEMKEinventors
Life of this patent
Estimating the term…
Abstract
Described herein are methods for obtaining enucleated cells from nucleated cells. Also described herein are methods for cell processing, including providing a composition containing nucleated cells and enucleating at least a portion of the nucleated cells to produce an enucleated cell fraction. Also described herein are methods for cell processing, including expressing the heterologous gene product. Also provided are pharmaceuticals compositions comprising an enucleated cell.
Claims118
A method of delivering a therapeutic agent to a target cell of a subject, the method comprising introducing a plurality of enucleated cells comprising the therapeutic agent to the subject or a sample of the subject in vivo or ex vivo under conditions sufficient to deliver the therapeutic agent to the target cell of the subject, wherein the plurality of enucleated cells is obtained from a cryopreserved composition or a cryohibernated composition, and wherein the therapeutic agent is delivered to the target cell in an amount that is greater than or equal to about an amount of the therapeutic agent delivered to an otherwise comparable target cell of the subject by otherwise comparable enucleated cells that were not cryopreserved or not cryohibernated.
A composition, comprising:
- a plurality of enucleated cells formulated from a cryopreserved composition or a cryohibernated composition, wherein the cryopreserved composition or the cryohibernated composition comprises the plurality of enucleated cells that are cryopreserved or cryohibernated, wherein at least a subset of the plurality of enucleated cells comprises (i) a therapeutic agent, and (ii) intracellular organelles sufficient to release the therapeutic agent in vivo or ex vivo in an amount that is greater than or equal to about an amount of the therapeutic agent released by otherwise identical enucleated cells that were not cryopreserved or not cryohibernated.
Dependencies are inferred from the claim text.