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US 2026/0015674 A1Published application
Use of pa-tn5 to generate dna libraries
OwnerZhiguo Zhang, Regina Santella +2inventors
InventorsZhiguo Zhang, Regina Santella, Hui Zhou, Hui-Chen Wu
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Abstract
Disclosed herein are methods of preparing a DNA library using a pA-Tn5 transposase, methods of identifying a modification of interest in DNA using a pA-Tn5 transposase, and kits for carrying out the disclosed methods.
Claims20
A method of preparing a DNA library from isolated DNA, the method comprising:
- incubating the isolated DNA with a fusion protein comprising protein A and a Tn5 transposase (pA-Tn5) to thereby generate DNA fragments, and
- isolating the DNA fragments to form the DNA library.
A method of identifying a modification of interest in isolated DNA, the method comprising:
- incubating the isolated DNA with pA-Tn5 to thereby generate DNA fragments;
- isolating DNA fragments having the modification of interest;
- and
- identifying the modification of interest from the DNA fragments.
1 more element
A method of diagnosing cancer in an individual, the method comprising:
- incubating DNA obtained from the individual with pA-Tn5 to thereby generate DNA fragments;
- isolating DNA fragments having a modification of interest;
- and
- identifying the position of the modification of interest from the DNA fragments, thereby diagnosing cancer.
1 more element
Dependencies are inferred from the claim text.