US 2026/0015664 A1 · METHODS FOR DETERMINATION AND MONITORING OF TRANSPLANT REJECTION BY MEASURING RNA

Abstract

The present disclosure provides methods for preparation and analysis of biological samples of transplant recipients or subjects suffering from a disease or disorder, wherein the methods comprise extracting fragmented or intact RNA (such as mRNA or miRNA) derived from sample of the transplant recipient or subject suffering from a disease or disorder, wherein the extracted RNA comprises target RNA molecules preselected to enable assessment of transplant rejection. The detection of the target RNA molecules and total amount of RNA derived from the donor organ can be used to determine and/or monitoring transplant rejection.

Claims134

Dependent claims

A method of preparing a composition of amplified nucleic acids derived from a sample obtained from a transplant recipient useful for determining transplant rejection, comprising:

a) extracting fragmented or intact RNA derived from the sample obtained from the transplant recipient, wherein the extracted RNA comprises donor- and/or recipient-derived RNA;
b) preparing a composition of amplified nucleic acids from the RNA by performing reverse transcription of the RNA to produce complementary DNA (cDNA) followed by multiplex targeted amplification to obtain amplicons of a plurality of target RNA molecules in a single reaction mixture, wherein the plurality of target RNA molecules is indicative of transplant rejection or organ health.

Dependencies are inferred from the claim text.

Methods for determination and monitoring of transplant rejection by measuring rna

Life of this patent

Methods for determination and monitoring of transplant rejection by measuring rna

Life of this patent

Abstract

Claims134