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US 2026/0015656 A1Published application
Determination of polymorphisms using isothermal nucleic acid amplification
AssigneeAbbott Diagnostics Scarborough, Inc.
InventorsMatthew Simon Forrest, Niall A. Armes
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Abstract
This invention relates to methods and compositions for detecting a polymorphism in a target nucleic acid sequence using isothermal nucleic acid amplification. More specifically, the present invention relates to using recombinase polymerase amplification (RPA) or Nicking and Extension Amplification Reaction (NEAR) to detect single nucleotide polymorphisms in a target nucleic acid sequence.
Claims20
A composition for detecting a polymorphism in a target nucleic acid sequence comprising:
- (i) a first primer and second primer for amplifying the target nucleic acid sequence;
- (ii) one or more recombinase(s);
- (iii) one or more polymerase(s);
- (iv) an agent capable of cleaving double stranded nucleic acid at a target cleavage sequence.
1 more element
A method of determining the presence or absence of a polymorphism in a target nucleic acid sequence, comprising:
- (a) contacting the sample comprising the target nucleic acid sequence with a mixture comprising
- a first primer and a second primer for amplifying the target nucleic acid sequence;
- a recombinase,
- a polymerase, and
- an agent capable of cleaving double-stranded nucleic acid at a target cleavage sequence,
- (b) performing a nucleic acid amplification reaction of the mixture for production of nucleic amplification products in the mixture;
- (c) monitoring the rate of increase of nucleic acid amplification products in the mixture;
- wherein an exponential rate of increase of nucleic acid amplification products indicates the presence or absence of the polymorphism in the target nucleic acid sequence.
5 more elements
Dependencies are inferred from the claim text.