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US 2026/0015604 A1Published application
Microfluidic apparatus and methods of use thereof
AssigneeNutcracker Therapeutics, Inc.
InventorsBenjamin Eldridge, Ximiao Wen
Life of this patent
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Abstract
Apparatuses and methods are described herein for processing polynucleotides in a scaled path environment. The apparatuses include optical sensors to monitor operations and to track material usage for good manufacturing practice.
Claims21
1-97. (canceled)
A microfluidic apparatus for forming a therapeutic polynucleotide, the apparatus comprising:
- (a) a seating mount for removably holding a microfluidic path device;
- (b) a plurality of pressure lines, each pressure line being operable to provide pneumatic pressure to a microfluidic path device held in the seating mount;
- (c) a plurality of fluidic lines, each fluidic line being operable to provide fluid to a microfluidic path device held in the seating mount to form a closed fluid path;
- and
- (d) a controller comprising one or more processors, the one or more processors programmed to drive fluidic movement in a flow, from one region of the microfluidic path device to at least one other region of the microfluidic path device when the microfluidic path device is held in the seating mount, to thereby provide one or more of the following:
- (i) direct the synthesis of a synthetic template in one or more microfluidic path devices held in the seating mount,
- (ii) direct an in vitro transcription (IVT) reaction using the template to form a therapeutic polynucleotide in one or more microfluidic path devices held in the seating mount, or
- (iii) direct purification of the therapeutic polynucleotide in one or more microfluidic path devices held in the seating mount.
5 more elements
A microfluidic apparatus for forming a therapeutic polynucleotide, the apparatus comprising:
- (a) a seating mount for removably holding a microfluidic path device;
- (b) a plurality of pressure lines, each pressure line being operable to provide pneumatic pressure to a microfluidic path device held in the seating mount;
- (c) a plurality of fluidic lines, each fluidic line being operable to provide fluid to a microfluidic path device held in the seating mount to form a closed fluid path;
- (d) a microfluidic path device configured to fit in the seating mount, the microfluidic path device including:
- (i) a first chamber,
- (ii) a second chamber, and
- (iii) a channel providing a path for communication of fluid from the first chamber to the second chamber;
- and
- (e) a controller comprising one or more processors, the or more processors programmed to drive fluidic movement in a flow from the first chamber to the second chamber via the channel to thereby form a therapeutic polynucleotide in the microfluidic path device while the microfluidic path device is held in the seating mount.
6 more elements
An apparatus for forming a therapeutic polynucleotide, the apparatus comprising:
- (a) at least one process chip, including:
- (i) a first chamber,
- (ii) a second chamber, and
- (iii) a channel providing a path for communication of fluid from the first chamber to the second chamber
- (b) a plurality of fluid containers;
- (c) a seating mount removably holding the at least one process chip;
- (d) a plurality of pressure lines, each pressure line being operable to provide pneumatic pressure to the at least one process chip held in the seating mount;
- (e) a plurality of fluidic lines coupled with the plurality of fluid containers, each fluidic line being operable to provide fluid to the at least one process chip held in the seating mount such that the plurality of fluidic lines, the plurality of fluid containers, and the at least one process chip collectively form a closed fluid path so that fluid within the closed fluid path is isolated from atmosphere;
- and
- (f) a controller comprising one or more processors, the one or more processors programmed to drive fluidic movement in a flow, from the first chamber to the second chamber when the at least one process chip is held in the seating mount, to thereby provide one or more of the following:
- (i) direct the synthesis of a synthetic template in the at least one process chip held in the seating mount,
- (ii) direct an in vitro transcription (IVT) reaction using the template to form a therapeutic polynucleotide in the at least one process chip held in the seating mount, or
- (iii) direct purification of the therapeutic polynucleotide in the at least one process chip held in the seating mount.
10 more elements
Dependencies are inferred from the claim text.