Improved loading of molecules and complexes to reaction sites
AssigneePacific Biosciences of California, Inc.
InventorsCarl WASSERMANN, Richa Pathak, Aparna Vedula, Alexander Plumridge, Thang Pham
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Abstract
Disclosed are methods for directly loading samples to reaction sites on a surface of a substrate without any step of prewetting the surface. The methods result in a reduced sample input amount by at least 2- to 10-fold compared to the conventional protocol that requires the prewetting step.
Claims36
A method of loading polymerase enzyme complexes into a plurality of nanoscale wells, the method comprising contacting a loading solution to a surface of a substrate comprising a plurality of nanoscale wells, wherein the loading solution comprises:
- (a) one or more polymerase enzyme complexes comprising a template nucleic acid and a polymerase enzyme;
- (b) one or more nonionic surfactants;
- and
- (c) one or more crowding agents.
A loading solution for loading polymerase enzyme complexes into a plurality of nanoscale wells, the loading solution comprising:
- (a) one or more nonionic surfactants;
- and
- (b) one or more crowding agents.
Dependencies are inferred from the claim text.