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US 2026/0015575 A1Published application
Methods of automated embryoid body embedding in hydrogel using separation well microplate
AssigneeMolecular Devices (Austria) GmbH
InventorsJosef Atzler, Sara Sofia Deville
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Abstract
Methods are provided for automated embedding of embryoid bodies in a hydrogel, media exchange, culturing organoids, and monitoring the development of organoids such as brain organoids. Methods for automated testing of compounds and toxicity effects are also provided.
Claims24
A method for culturing of organoids, the method comprising
- adding an embryoid body into a first media within a primary well of a separation well microplate comprising a plurality of well units, each well unit comprising a primary well, a secondary well, and one or more microchannels connecting the primary well to the secondary well;
- removing the first media from the well unit via the secondary well;
- adding a liquid hydrogel to encapsulate the embryoid body;
- incubating the separation well microplate over a first period of time to solidify the liquid hydrogel encapsulating the embryoid body into a solidified hydrogel;
- and
- adding a second media to the secondary well to detach the solidified hydrogel encapsulating the embryoid body from the plate and suspend in the second media.
3 more elements
Dependencies are inferred from the claim text.