Aseptic pinching device and operating method thereof

An operating method of an aseptic pinching device, comprising steps of:

• a) providing two half components, wherein each one of the two half components has a base structure, a first buckle structure disposed on a first side of the base structure, and a second buckle structure disposed on a second side of the base structure opposite to the first side, and the two half components are identical;
• and
• b) flipping one of the half components 180 degrees relative to the other of the half components such that one of the half components is brought toward the other of the half components to engage each other, wherein a diagonal space is formed by the base structure of one of the half components and the base structure of the other of the half components, and the diagonal space is configured to accommodate a sleeve to pinch the sleeve between the two half components.