REGULATORY SEQUENCES COMPRISING MicroRNA TARGET SITES
AssigneeSensorion
InventorsLaurent DÉSIRÉ, Patrice VIDAL, Arnaud GIESE, Rafik BOUDRA
Life of this patent
Estimating the term…
Abstract
An isolated nucleic acid sequence including at least two copies of a miRNA target site of the miR183 family having a sequence as set forth in SEQ ID NO: 1, SEQ ID NO: 21, or SEQ ID NO: 24, or a sequence having at least 90% identity with any one of SEQ ID NO: 1, SEQ ID NO: 21, or SEQ ID NO: 24. Also a vector including a regulatory element having at least one copy of this miRNA target site of the miR183 family. The isolated nucleic acid sequence and the vector may be particularly useful for controlling the expression of a gene of interest, for example when designing and developing gene therapies.
Claims21
1-15. (canceled)
An isolated nucleic acid sequence comprising at least two copies of a miRNA target site of the miR183 family, wherein said miRNA target site has a sequence as set forth in SEQ ID NO:
- 1, SEQ ID NO:
- 21, or SEQ ID NO:
- 24, or a sequence having at least 90% identity with any one of SEQ ID NO:
- 1, SEQ ID NO:
- 21, or SEQ ID NO:
- 24.
An expression cassette comprising a promoter, a gene of interest, and a regulatory element comprising at least one copy of a miRNA target site of the miR183 family, or a vector comprising said expression cassette, wherein said miRNA target site has a sequence as set forth in SEQ ID NO:
- 1, SEQ ID NO:
- 21, or SEQ ID NO:
- 24, or a sequence having at least 90% identity with any one of SEQ ID NO:
- 1, SEQ ID NO:
- 21, or SEQ ID NO:
- 24.
A vector comprising a regulatory element comprising at least one copy of a miRNA target site of the miR183 family, wherein said miRNA target site has a sequence as set forth in SEQ ID NO:
- 1, SEQ ID NO:
- 21, or SEQ ID NO:
- 24, or a sequence having at least 90% identity with any one of SEQ ID NO:
- 1, SEQ ID NO:
- 21, or SEQ ID NO:
- 24.
Dependencies are inferred from the claim text.