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US 2026/0002191 A1Published application
Nad(p)- dependent responsive enzymes, electrodes and sensors, and methods for making and using the same
AssigneeAbbott Diabetes Care Inc.
InventorsTianmei OUYANG, Benjamin J. FELDMAN
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Abstract
NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof, an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.
Claims27
An analyte sensor system, comprising
- a transcutaneous electrochemical analyte sensor for detecting D-3-hydroxybutyrate in vivo, the sensor comprising:
- a working electrode configured to generate a signal associated with the level of D-3-hydroxybutyrate;
- an enzyme composition deposited on a surface of the working electrode, wherein the enzyme composition comprises:
- a. nicotinamide adenine dinucleotide (phosphate) (NAD(P)+) or derivative thereof;
- b. D-3-hydroxybutyrate dehydrogenase;
- c. diaphorase;
- d. an electron transfer agent;
- e. a polymer, wherein the NAD(P)+ or derivative thereof is not covalently bonded to the polymer;
- and
- a mass transport limiting membrane disposed over the enzyme composition to limit flux of D-3-hydroxybutyrate to the working electrode;
- sensor electronics;
- and
- a receiver.
10 more elements
An analyte sensor system, comprising
- a transcutaneous electrochemical analyte sensor for detecting D-3-hydroxybutyrate in vivo, the sensor comprising:
- a working electrode configured to generate a signal associated with the level of D-3-hydroxybutyrate;
- an enzyme composition deposited on a surface of the working electrode, wherein the enzyme composition comprises:
- a. nicotinamide adenine dinucleotide (phosphate) (NAD(P)+) or derivative thereof;
- b. D-3-hydroxybutyrate dehydrogenase;
- c. diaphorase;
- d. a polymeric redox mediator;
- and
- a mass transport limiting membrane disposed over the enzyme composition to limit flux of D-3-hydroxybutyrate to the working electrode;
- and
- sensor electronics;
- and
- a receiver.
10 more elements
Dependencies are inferred from the claim text.