US 2026/0002182 A1 · ENGINEERING BACTERIA EXPRESSING ASPARTATE DEHYDROGENASE AND METHOD FOR PRODUCING VITAMIN B5 BY FERMENTATION

Abstract

The present application relates to the field of microorganisms, and specifically relates to Escherichia coli expressing aspartate dehydrogenase aspDH and a method for producing vitamin B5 by fermentation. Overexpression of the aspDH gene shows the best result by comparing the fermentation yield of VB5. Compared with the highly polluting chemical method for the production of vitamin B5, the biological method for the production of vitamin B5 of the present application has the advantages of renewable raw materials, easy treatment and resource utilization of waste residue, waste water and waste gas, and thus can be used in practice for the industrial production of vitamin B5, which is of significant application value.

Claims11

Dependent claims

An application of enhanced expression of an aspartate dehydrogenase gene aspDH in the production of vitamin B5;

preferably, the aspartate dehydrogenase gene aspDH is derived from Delftia sp. Csl-4.

A host, wherein the host expresses an aspartate dehydrogenase gene aspDH;

preferably, the aspartate dehydrogenase gene aspDH is derived from Delftia sp. Csl-4;
preferably, the aspartate dehydrogenase gene aspDH has:
(I) a nucleotide sequence shown as SEQ ID No. 56;
or
(II) a nucleotide sequence obtained by substituting, deleting or adding one or more bases to the nucleotide sequence shown as (I), and having the same or similar function as the nucleotide sequence shown as (I);
or
(III) a nucleotide sequence having at least 80% homology to the nucleotide sequence shown as (I) or (II).

4 more elements

Dependencies are inferred from the claim text.

Facts

Application

18/841,611Filed 17 Feb 2023

Priority

CN 202210214588.57 Mar 2022

Published

1 Jan 2026

Term ends

Not estimatedThe source records don’t support an estimate.

Classification16 codes

C12P 13/02
Preparation of nitrogen-containing organic compounds: amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds…
C07K 14/195
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof: from bacteria
C12N 1/20
Microorganisms; Compositions thereof ; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of…:…
C12N 9/0006
Oxidoreductases: acting on CH-OH groups as donors

Engineering bacteria expressing aspartate dehydrogenase and method for producing vitamin b5 by fermentation

Life of this patent

Engineering bacteria expressing aspartate dehydrogenase and method for producing vitamin b5 by fermentation

Life of this patent

Abstract

Claims11