Application of aspartate decarboxylase in the production of vitamin b5 by fermentation

Abstract

The present application relates to the field of microorganisms and specifically relates to highly active aspartate decarboxylases for the production of vitamin B5. In the present application, the L-aspartate α-decarboxylase derived from Bacillus licheniformis was screened, the PanD with significantly higher activity which catalyze the production of β-alanine than other orgins of PanD. The engineering bacterium for the fermentative production of vitamin B5 was constructed by applying PanD derived from B. licheniformis. The bottleneck of β-alanine metabolism in the biosynthesis of vitamin B5 was lifted. Compared with the highly polluting chemical method for the production of vitamin B5, the biological method for the production of vitamin B5 of the present application has the advantages of renewable raw materials, easy treatment and resource utilization of waste residue, waste water and waste gas, and thus can be used in practice for the industrial production of vitamin B5, which is of significant application value.

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