Heterologous protease expression for improving alcoholic fermentation
AssigneeDanstar Ferment AG
InventorsTrisha Barrett, Charles F. Rice, Aaron Argyros
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Abstract
The present disclosure relates to proteases for improving alcoholic fermentation. The proteases are expressed from a recombinant host cell. The present disclosure also provides a population of recombinant host cells expressing an heterologous protease that can be used in combination with recombinant host cells expressing an heterologous glucoamylase and/or an heterologous glycerol reduction system.
Claims16
A process for promoting ethanolic fermentation, the process comprising fermenting a medium with a recombinant yeast host cell, wherein the recombinant yeast host cell comprises a first genetic modification allowing the expression of a heterologous protease, wherein the heterologous protease is:
- a) a polypeptide having the amino acid sequence of SEQ ID NO:
- 14;
- b) a variant having at least 70% identity to the polypeptide of a) and exhibiting proteolytic activity;
- or
- c) a fragment having at least 70% identity to the polypeptide of a) or the variant of b) and exhibiting proteolytic activity.
Dependencies are inferred from the claim text.