Back to results
US 2026/0002108 A1Published application
Factory-on-a-chip for production of biologically derived medicines/biopharmaceuticals/biologics/ biotherapeutics
AssigneeUniversity of Maryland, Baltimore County
InventorsGOVIND RAO, YORDAN KOSTOV, ABHAY ANDAR, MUSTAFA AL-ADHAMI, SEVDA DELDARI, DOUGLAS D. FREY
Life of this patent
Estimating the term…
Abstract
The present invention provides for a fully integrated microfluidic system capable of producing single-dose amounts of biotherapeutics at the point-of-care wherein protein production, purification and product harvest are all integrated as a single microfluidic device which is portable and capable of continuous-flow production of biotherapeutics at the microscale using a cell-free reaction system.
Claims16
A method of preparing a therapeutic protein on demand, the method comprising:
- synthesizing the therapeutic protein using a factory-on-a-chip microfluidic device comprising at least three separate units, wherein the separate units comprise:
- (i) a microfluidic bioreactor unit equipped with a continuous collection channel for a synthesizing a crude protein within the microfluidic bioreactor unit, wherein the microfluidic bioreactor unit comprises cell extracts and a reaction mixture for expression of the crude protein;
- (ii) a microfluidic mixer/de-bubbler unit communicatively connected to the microfluidic bioreactor unit to dilute the crude protein and remove any air bubbles during mixing;
- and
- (iii) a microfluidic purification unit communicatively connected to, and downstream of, the microfluidic mixer/de-bubbler unit for capturing the crude protein and providing the purified therapeutic protein, wherein the microfluidic purification unit comprises at least one purification column comprising chromatography resin.
2 more elements
Dependencies are inferred from the claim text.