Dissociation Method and System of Deprotonated Peptides with Fragile Moieties
AssigneeDH Technologies Development Pte. Ltd.
InventorsTakashi BABA, Haichuan LIU, Yuzhuo ZHANG
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Abstract
A method for mass spectrometric analysis of a peptide having at least one fragile moiety includes using electrospray ionization to generate a negatively charged ion of said peptide, trapping and cooling the negatively charged peptide ion in a radiofrequency (RF) ion trap containing a cooling buffer gas, and exposing said cooled, trapped peptide ion to an electron beam so as to cause negative electron activated dissociation (negative EAD) of the negatively charged peptide ion to generate a plurality of fragment ions.
Claims20
A method for mass spectrometric analysis of a peptide having at least one fragile moiety, comprising:
- using electrospray ionization to generate a negatively charged ion of said peptide,
- trapping and cooling the negatively charged peptide ion in a radiofrequency (RF) ion trap containing a cooling buffer gas, and
- exposing said cooled, trapped peptide ion to an electron beam so as to cause negative electron activated dissociation (negative EAD) of the negatively charged peptide ion to generate a plurality of fragment ions.
A mass spectrometer, comprising:
- an ion source configured to ionize a plurality of peptides that include a fragile moiety thereby generating a plurality of peptide ions that include a fragile moiety;
- and
- a chamber including:
- an ion trap that includes a buffer gas and is configured to trap the peptide ions, and
- an electron source configured to generate a plurality of electrons in the form of an electron beam and configured to introduce the electron beam into the ion trap,
- wherein molecules of the buffer gas cool the peptide ions within the ion trap, and
- wherein the electron beam interacts with at least a portion of the peptide ions to cause negative EAD thereby generating a plurality of fragment ions with a fragile moiety.
A chamber for use in a mass spectrometer, comprising:
- an ion trap including:
- a plurality of negatively charged peptide ions that include a fragile moiety, and
- a buffer gas configured to reduce a vibrational state of the peptide ions;
- and
- an electron source that is external from the ion trap and is configured to introduce an electron beam into the ion trap, wherein the electron beam interacts with the peptide ions to generate a plurality of fragment ions with a fragile moiety.
Dependencies are inferred from the claim text.