Differential separation process with controlled parameters relevant to solid and liquid phase

A generalized separation process is operated to eliminate displacement zone in chromatographic sequential operation via single cell for separating at least one desired component from a liquid solution mixture containing one component and at least other component named as mobile phase feed solution via beginning to obtain a characteristic separation profile by carrying out a new mass transfer equilibrium contact method transmitting liquid feed solution mobile phase in contacting with a solid phase resin/adsorbent packing material, so that at least one of component contained in said liquid feed solution can be adsorbed onto resin/adsorbent, subsequently via said new mass transfer equilibrium contact method to transmit at least of second and third mobile phases of various kind liquid solutions comprising predetermined composition in sequential contact with said resin/adsorbent, so that, at least two of components are desorbed one after thereof;

• and wherein generalized separation process comprise following hybrid embodiments as general procedures of new mass transfer equilibrium contact method between solid and liquid mobile phase employed by an apparatus operating via differential set up and through single stage recycle protocol;
• thus
• A. starting with determining optimal full-strength bonding capacity of resin/adsorbent with a prefixed feed solution throughput and such solid phase amount is equivalent to mass transfer zone in chromatography to carry out above said mass transfer mechanism;
• wherein retaining predetermined solid phase resin/adsorbent amount distributed equally in a bundled group of predetermined quantity of columns, each column having an inlet on top side and an outlet on another side with bottom meshed filter to contain equal amount of said resin/adsorbent from being drained;
• such bundled group of columns performing like partially fluidized beds as a whole unit being named as aforesaid single cell hereinafter;
• wherein each column retaining equal amount of resin/adsorbent solid material as plurality of columns installed in said single cell and sum amount of each disposed resin/adsorbent being corresponding to resin/adsorbent installed as mass transfer zone in chromatography to fully saturated with prefixed feed solution throughput;
• the liquid inlet of cell is from top and liquid outlet of cell is from bottom;
• 1. intermittently delivering predetermined amounts of mobile phase liquid material through predetermined type of liquid dispenser in portion as predetermined input format dose dropping amid predetermined first time period including mobile phase feed solution, at least of second and third mobile phases of various kind of liquid solutions comprising predetermined composition in sequential contact with said resin/adsorbent to promote predetermined new mass transfer equilibrium contact method either promoting adsorption of dissolved components onto said resin/adsorbent or eluting adsorbed components from said resin/adsorbent;
• 2. intermittently supplying broad range pressurized inert gas to a cell top side following each delivery of said mobile phase dose means amid predetermined second time period for maintaining sufficient pressure to force prompt draining such mobile phase liquid percolate through said solid phase material to complete expected new mass transfer equilibrium contact method;
• 3. maintaining a vacuum environment on the other side of said solid phase material installed in said cell meanwhile amid spent time during step (2) to maintain resin/adsorbent material in a semi-dry status;
• wherein said broad range pressurized inert gas being supplied from cell top and whereas vacuum meanwhile being exerted from cell bottom;
• 4. collecting most of treated mobile phase liquid material meanwhile amid spent time during step (2) from the outlet of cell bottom;
• 5. defining in sum of spent time amid step (1) through step (4) as minimal time interval;
• wherein said generalized separation process further proceed
• B. via aforesaid new mass transfer equilibrium contact method to obtain a characteristic separation profile for particular separation system wherein amount of said resin/adsorbent is predetermined and being maintained in said semi-dry status as full-strength bonding capacity with a predetermined solution amount comprising a plurality of components and disposed in equal sub-amount in a plurality of column in said single cell, wherein a method for separating said plurality of components contained in at least one of first mobile phase stream comprising a liquid solution of said components by contacting a solid phase resin/adsorbent with the at least one mobile phase stream in sequence, so that at least one of component being adsorbed by the resin/adsorbent, and wherein mobile phase being delivered through predetermined type of liquid dispenser and combination of selected liquid input format, wherein input S-I mode is defined as that predetermined amounts of same mobile phase parametric condition solution is delivered within the said minimal time interval into said cell, and wherein mobile phase being delivered through predetermined type of liquid dispenser and selected liquid input format, wherein input I-I is defined as that predetermined amounts of “discrete increments” of mobile phase parametric condition solutions are delivered into said cell within said minimal time interval, by options of said input S-I, wherein
• a. contacting said resin/adsorbent with at least one amount of first mobile phase stream illustrated in step (1) through step (5) of step A and define total cumulated minimal time interval as first mobile phase zone;
• subsequently
• b. contacting said resin/adsorbent with at least one amount of second mobile phase stream in sequence, each second stream comprising liquid eluent of predetermined composition to reach expected new mass transfer equilibrium contact method such that at least one of component is individually desorbed from the resin/adsorbent in sequence illustrated in step (1) through step (5) of step A and total cumulated minimal time interval is defined as second mobile phase zone;
• subsequently;
• c. contacting said resin/adsorbent with at least one of third mobile phase stream in sequence, each third stream comprising liquid eluent of predetermined composition to reach expected new mass transfer equilibrium contact method such that at least one of component is individually desorbed from the resin/adsorbent in sequence illustrated in step (1) through step (5) of step A and total cumulated minimal time interval is defined as third mobile phase zone;
• d. repeating step (c) of step B by contacting said resin/adsorbent with at least one amount of fourth mobile phase stream if remaining components still remained been adsorbed with said resin/adsorbent, such that components can be eluted by predetermined composition to reach expected new mass transfer equilibrium contact method such that remaining components is individually desorbed from the resin/adsorbent in sequence;
• otherwise,
• e. contacting said resin/adsorbent with at least one amount of at least one of said drained and collected first mobile phase liquid stream in step (a) of step B to the inlet of the cell in sequence illustrated in in step (1) through step (5) of step A and total cumulated minimal time interval is defined as last mobile phase zone;
• and
• f, wherein lastly to integrate all defined zones in sequential prevail with respect to mobile phase parametric condition in time domain to conclude obtaining characteristic separation profile for particular separation system via input S-I;
• C. further wherein said new mass transfer equilibrium contact method is to deliver mobile phase in format by options of said input I-I as following;
• 1) contacting said resin/adsorbent with at least one amount of first mobile phase stream illustrated in step (1) through step (5) of step A and define total cumulated minimal time interval as first discrete mobile phase zone;
• subsequently repeating this step by increasing a discrete increment of mobile phase parametric conditions until all of said at least one amount of first mobile phase stream being adjusted with preset incremental discrete delivered in sequence and total cumulated minimal time interval is defined as multiple first discrete mobile phase zone named in sequential order corresponding to respective delivered mobile phase parametric condition;
• subsequently
• 2) contacting said resin/adsorbent with at least one amount of second mobile phase stream in sequence, each second stream comprising liquid eluent of predetermined composition in discrete increment of parametric condition to reach expected new mass transfer equilibrium contact method such that at least one of component is individually desorbed from the resin/adsorbent in sequence illustrated in step (1) through step (5) of step A and total cumulated minimal time interval is defined as second discrete mobile phase zone;
• repeating this step by increasing a discrete increment of mobile phase parametric conditions until all of said at least one amount of second mobile phase stream being adjusted with preset incremental discrete delivered in sequence and total cumulated minimal time interval is defined as multiple second discrete mobile phase zone named in sequential order corresponding to respective delivered mobile phase parametric condition;
• subsequently
• 3) contacting said resin/adsorbent with at least one amount of third mobile phase stream in sequence, each third stream comprising liquid eluent of predetermined increment of mobile phase parametric composition to reach expected new mass transfer equilibrium contact method such that at least one of component is individually desorbed from the resin/adsorbent in sequence illustrated in step (1) through step (5) of step A and total cumulated minimal time interval is defined as third discrete mobile phase zone;
• repeating this step by increasing a discrete increment of mobile phase parametric conditions until all of said at least one amount of third mobile phase stream being adjusted with preset incremental discrete delivered in sequence and total cumulated minimal time interval is defined as multiple third discrete mobile phase zone named in sequential order corresponding to respective delivered mobile phase parametric condition;
• subsequently
• 4) contacting said resin/adsorbent with at least one amount of fourth mobile phase stream if remaining components still been adsorbed with said resin/adsorbent, such that components can be eluted by predetermined composition with discrete increment of mobile phase parametric condition to reach expected new mass transfer equilibrium contact method such that remaining components is individually desorbed from the resin/adsorbent in sequence;
• 5) otherwise, contacting said resin/adsorbent with at least one amount of said drained and collected first mobile phase liquid stream in step (1) of this input format I-I amid predetermined first time period to the inlet of said cell in sequence illustrated in step (1) through step (5) of step A and total cumulated minimal time interval is defined as last mobile phase zone;
• and
• 6) wherein lastly to integrate all defined zones in sequential prevail with respect to mobile phase parametric condition in time domain to conclude obtaining characteristic separation profile for particular separation system via input I-I;
• further wherein
• D. an apparatus employed for integrating multiple modules contained in a closed loop connected in sequence yet functioning independently as a whole unit, such apparatus comprising at least one of following module, wherein
• 1) providing upstream holding tanks module, wherein
• a. having a plurality of holding tanks disposed in an organized order setting inside an insulated heat media circulation jacket to maintain whole plurality in a selected temperature range;
• b. each said holding tank of whole plurality having an inlet liquid conduct extended outside upward of said jacket to receive liquid via an preferred mechanical device opened flipper, named as flipper 1 hereinafter disposed about bottom inside of said liquid conduct;
• c. each of said whole plurality holding tanks having an outlet liquid conduct extended outside downward of said jacket installed with a preferred pressure activated check valve disposed about top inside of said liquid conduct to hold the received liquid or to discharge liquid into following module amid exerted pressure;
• 2) providing separation module, wherein
• (a) single cell comprising predetermined quantity of columns orderly disposed inside said cell;
• each column having an top side inlet and a bottom side outlet with meshed filter to contain equal amount of resin/adsorbent material from being drained;
• (b) said single cell disposed inside an insulated heat media circulation jacket to maintain said cell in a selected temperature range;
• (c) said single cell top having an inlet liquid conduct as temporary transit reservoir extended outside upward of said heat media circulation jacket to receive particular liquid delivered from corresponding holding tank in said upstream holding tanks module through an opened flipper, named as flipper 3 hereinafter disposed around top inside of said liquid transit storage reservoir, through liquid pipe delivering respective liquid through predetermined type of liquid dispenser down below;
• such dispenser comprising of another preferred mechanical device flipper, named as flipper 4 hereinafter disposed inside in between bottom side of said temporary transit reservoir and top side of the liquid dispenser;
• (d) said single cell bottom being exposed to said vacuum environment containing a transit reservoir with its widely open top means to withdraw mist enriched inert gas via its gas exit pipe connect to a manifold to maintain said resin/adsorbent in a semi-dry status, meanwhile to affiliating liquid draining via disposed funneled shape liquid conduct through;
• (e) another opened mechanical flipper, names as flipper 65 hereinafter disposed inside bottom of said funnel conduct, into underneath temporary liquid reservoir;
• having a gas pipe connected with same manifold next to said mist enriched inert gas exit pipe means for supplying broad range pressurized inert gas via this manifold to shut off said flipper 65 soon said vacuum environment being turned off, so that pushing entire drained liquid into following module via a preferred pressurize activated check valve disposed around top inside of said bottom liquid conduct, wherein said inert gas manifold being disposed underneath said vacuum environment and being extended outside downward of said insulated heat media circulation jacket;
• 3) providing downstream holding tanks module, wherein
• (a) having a plurality of holding tank disposed in an organized order setting inside an insulated heat media circulation jacket to maintain whole plurality of holding tanks in a selected temperature range and each tank having an inlet liquid conduct extended outside upward of the insulated heat media circulation jacket;
• (b) each of said whole plurality holding tanks comprising an outlet liquid conduct installed with said preferred pressure activated check valve extended outside downward of said jacket means for discharging stored liquid via each means of liquid distribution;
• (c) having a liquid level sensor installed inside each of said whole plurality holding tanks to monitor predetermined liquid level of stored liquid within for following;
• (i) means such level sensor is to control delivering sufficient volume amount of particular solution via liquid conduct disposed on top of such holding tank to maintain a predetermined liquid level setting in respective holding tank and holding received respective liquid;
• (ii) means for at least one isolated product into respective assigned storage tank;
• (iii) means for discharging components in particular solution mixture as by product into respective assigned storage tank;
• and
• (iv) means for transmitting in part of separated component stored in respective holding tank in predetermined volume recycling back into each assigned holding tank in aforesaid upstream holding tanks module;
• further wherein;
• 4) providing inert gas supply module means for mobile phase liquid streams transmitting mechanism for close loop routing supplying broad range pressurized inert gas inasmuch as incorporating with liquid fluid transmitting among said upstream holding tanks module, separation module, and downstream holding tanks module, wherein such module contain closed vacuum environment loop, upstream broad range inert gas supplying loop, and downstream broad range inert gas supplying loop;
• (a) proceeding closed vacuum environment loop comprising following procedures,
• (i) said single cell bottom been exposed to said vacuum environment to affiliate dropped dose liquid draining, meanwhile to extracting mobile phase liquid enriched mist inert gas to maintain said resin/adsorbent in semi-dry status through means extracting mist enriched inert gas to dry inert gas via driving force exerted from central vacuum pump through gas pipe via a selected mist separator to recover such mist from enriched inert gas;
• (ii) meanwhile to create a heterogeneous contact as following dropped dose of liquid promptly sipping through stationary resin/adsorbent particles to meet criterion of said new mass transfer equilibrium contact method;
• (iii) amid whole time, dry inert gas exiting mist separator being combined with pressurized dry air and deployed through an inert gas generator to obtain fresh dry inert gas and stored in a steel tank vessel maintaining preferred broad range of pressure level inert gas ready deploying back to following modules;
• (b) proceeding upstream broad range inert gas supplying loop means for upstream holding tanks module and said single cell top region in separation module wherein comprising following procedures,
• (i) stop supplying broad pressure range of inert gas through its respective gas pipe disposed around each said holding tank top side to allow predetermined liquid volume transferred from assigned holding tank in said downstream holding tanks module via each said volumetric pump freely passing through top side liquid conduct;
• soon is completed;
• subsequently
• (ii) closing such flipper 1 via supplying broad pressure range of inert gas to push opening said pressure activated check valve to discharge received liquid into said cell top temporary transit reservoir located at top of said separation module;
• soon is completed;
• subsequently
• (iii) via means of alternatively and simultaneously supplying of broad range pressurized inert gas through gas pipe connected in between said flipper 3 and flipper 4 of said temporary transit reservoir and another gas pipe disposed next to said temporary transit reservoir to intermittently dose dropping in parts of received liquid out of temporary transit reservoir resulting as predetermined said liquid input format to promptly wet top portion and promptly sipping through resin/adsorbent bed to carry out expected new mass transfer equilibrium contact method;
• (c) proceeding downstream broad range inert gas supplying loop means for said single bottom region in separation module and downstream holding tanks module wherein comprising following procedures
• (i) during duration of exerted vacuum environment to continuously drain treated doses of liquid into underneath liquid reservoir and said preferred pressure activated check valve holding drained liquid within;
• soon as liquid draining of entire transmitted liquid being completed;
• subsequently;
• (ii) shutting off vacuum environment, supplying low range pressurized inert gas routing via its pipe disposed next to said vacuum exit pipe in same manifold to close said flipper 65 to allow entire collected liquid freely pushing through opened said pressure activated check valve through liquid line connected to assigned holding tank in said downstream holding tanks module;
• E. as illustrated wherein differential set-up protocols employed via illustrated in step (1) through step (5) of step A for general procedures of new mass transfer equilibrium contact method onto aforesaid predetermined cell construction of step A to carry out by predetermined combination of selected mobile phase between in-put S-I and in-put I-I format to obtain a characteristic separation profile for particular separation system between selected resin/adsorbent related with various kinds of mobile phase liquid material illustrated in step B and step C into aforesaid apparatus comprising said single cell to separate at least one desired component from a liquid solution amid in sum of accumulated multiple minimal time interval in time domain, wherein following is said differential set-up protocol employed onto said apparatus, wherein
• 1. determining optimal full-strength bonding capacity of said resin/adsorbent with a prefixed feed throughput and filling such resin/adsorbent amount into a said cell contain multiple column disposed within;
• 2. sequentially breaking down characteristic elution profile of each partial time required to spend for respective predetermined mobile phase solution, this including feed solution mobile phase, at least one of recycle liquid stream organized in sequential order each with particular solute composition dissolved in particular modified characteristic parameter eluent solution mixture capable of eluting specific adsorbed solute mixture component, and eluent liquid followed with pressurized inert gas;
• 3. divide each said partial time in step (2) by said minimal time interval to obtain the range cumulated minimal time interval as major minimal time interval disposed for feeding zone and other zones following same major time interval of most time-consuming zone;
• then
• 4. divide total input volume of such liquid by the number of cumulated minimal time interval to obtain the partial volume required for each spent of minimal time interval in respective zone;
• then, divides such said partial volume by a preselected number to further differentiate for multiple liquid doses to reflect general procedures of said new mass transfer equilibrium contact method sipping through resin/adsorbent in said single cell;
• 5. further divides said resin/adsorbent amount in step 1, which being derived from complete saturation with feed solution throughput, by a preselected number that corresponds to differential resin/adsorbent amount disposed in at least one column bundled in said single cell to receive in sequence the partial volume of such liquid;
• 6. sequentially allocate each said major time interval with respective mobile phase solution as the range of respective zone and allocate all zones into an endless format in time domain;
• wherein integrating all zones orderly arranged in sequential format representing a complete elution separation cycle via transmitting all kind of mobile phase with finite parameter solution into underneath stationary separation module via predetermined combination of selected mobile phase between in-put S-I between I-I format, wherein
• (a) including setting up at least one zone between selection of introducing at least one amount of first mobile phase stream and multiple first discrete mobile phase stream in contact with disposed resin/adsorbent packing material in time domain to proceed expected mass transfer contact as first mobile phase zone;
• (b) including setting up at least one zone between selection of introducing at least one amount of second mobile phase stream and multiple second discrete mobile phase stream in contact with disposed resin/adsorbent packing material in time domain to proceed expected mass transfer contact as second mobile phase zone;
• (c) including setting up at least one zone between selection of introducing at least one amount of third mobile phase stream and multiple third discrete mobile phase stream in contact with disposed resin/adsorbent packing material in time domain to proceed expected mass transfer contact as third mobile phase zone;
• (d) including setting up at least one zone between selection of introducing at least one amount of fourth, fifth, and so forth mobile phase stream and multiple fourth, fifth, and so forth discrete mobile phase stream in contact with disposed resin/adsorbent packing material in time domain to proceed expected mass transfer contact as corresponding fourth, fifth, and so forth mobile phase zone until all adsorbed being eluted in sequence;
• (e) including setting up at least one zone corresponding to introducing at least one of last mobile phase stream in contact with disposed resin/adsorbent packing material in time domain to proceed expected mass transfer contact as last mobile phase zone;
• (f) and a predetermined plurality of alternative recycled mobile phase streams back to predetermined abovementioned zone to replace solution disposed in aforesaid corresponding zone to enhance concentration of separated component and save consumption of respective solution;
• (g) thus, integrating whole spectrum of above said zones organized in sequential order representing such obtained characteristic separation profile being for eliminating chromatography displacement zone and further arranging such differential protocols;
• wherein
• F. single stage recycle protocol employed onto said apparatus to complete separation procedures containing following:
• 1) providing said upstream holding tanks module containing plurality of holding tanks disposed in an organized order setting inside an insulated heat media circulation jacket to maintain whole plurality in a selected temperature range;
• wherein:
• (i) at least one holding tank disposed in first mobile phase zone, each containing said at least one of predetermined between selection of first mobile phase stream and multiple first discrete mobile phase stream;
• and
• (ii) at least one holding tank disposed in second mobile phase zone, each containing said at least one of predetermined between selection of second mobile phase stream and multiple second discrete mobile phase stream;
• and
• (iii) at least one holding tank disposed in third mobile phase zone, each containing said at least one of predetermined between selection of third mobile phase stream and multiple third discrete mobile phase stream;
• and
• (iv) at least one holding tank disposed in fourth, fifth, and so forth mobile phase zone, each containing said at least one of predetermined between selection of fourth mobile phase stream and multiple fourth, fifth, and so forth discrete mobile phase stream;
• and
• (v) at least one holding tank disposed in last mobile phase zone, each containing said at least one of predetermined last mobile phase stream;
• and further
• 2) providing said downstream holding tanks module containing a plurality of holding tanks disposed in same organized order as corresponding each holding tank disposed in said upstream holding tanks module to receive drained corresponding liquid transmitted from;
• such plurality holding tanks setting inside an insulated heat media circulation jacket to maintain whole plurality in a selected temperature range;
• wherein
• (i) at least one holding tank assigned for receiving in corresponding with liquid transmitted from particular holding tank disposed in said first mobile phase zone of upstream holding tanks module of said at least one of predetermined between selection of first mobile phase stream and multiple first discrete mobile phase stream in contact with resin/adsorbent solid phase packing material to attain new mass transfer equilibrium contact method;
• and
• (ii) at least one holding tank assigned for receiving in corresponding with liquid transmitted from particular tank disposed in said second mobile phase zone of upstream holding tanks module of said at least one of predetermined between selection of second mobile phase stream and multiple second discrete mobile phase stream in contact with resin/adsorbent solid phase packing material to attain new mass transfer equilibrium contact method;
• and
• (iii) at least one holding tank assigned for receiving in corresponding with liquid transmitted from particular tank disposed in said third mobile phase zone of upstream holding tanks module of said at least one of predetermined between selection of third mobile phase stream and multiple third discrete mobile phase stream in contact with resin/adsorbent solid phase packing material to attain new mass transfer equilibrium contact method;
• and
• (iv) at least one holding tank assigned for receiving in corresponding with liquid transmitted from particular tank disposed in said fourth, fifth, and so forth mobile phase zone of upstream holding tanks module of said at least one of predetermined between corresponding selection of fourth, fifth, and so forth mobile phase stream and multiple fourth, fifth, and so forth discrete mobile phase stream in contact with resin/adsorbent solid phase packing material to attain new mass transfer equilibrium contact method;
• and
• (v) at least one holding tank assigned for receiving in corresponding with liquid transmitted from particular tank disposed in said last mobile phase zone of upstream holding tanks module of said at least one of predetermined last mobile phase stream in contact with resin/adsorbent solid phase packing material to wash off retained resin/adsorbent solid packing material;
• further
• 3) providing said separation module comprising said single cell to retain predetermined solid phase resin/adsorbent as sum amount to fully saturated with prefixed feed solution throughput as mass transfer zone in chromatography and to distribute equal sub-portion amount in a plurality of each column having an inlet on top side and an outlet on bottom meshed filter to contain disposed resin/adsorbent from being drained;
• said single cell is disposed in an insulated heat media circulation jacket to maintain in a selected temperature range;
• said single cell further proceeding via said new mass transfer equilibrium contact method to carry out together with said mobile phase liquid streams transmitting mechanism to orderly deliver in sequence of aforesaid each mobile phase liquid stored in respective holding tank disposed corresponding zone in said upstream holding tanks module in a format of predetermined volume amount in sequential dose drop into said separation module to produce expected new mass transfer equilibrium contact method, and after each drop dose of sequential liquid delivery following repeated procedures soon predetermined amount of liquid solution being transmitted into said temporary transit reservoir disposed top portion of assigned single cell being completed, wherein
• (a) intermittently deliver through means of alternated supplying between two separated broad range of pressurized inert gas routings from said single cell top portion to force draining of dose dropped liquid promptly sipping through said resin/adsorbent to complete said new mass transfer equilibrium contact method between two phases to promote adsorption of components onto said resin/adsorbent or eluting adsorbed component from said resin/adsorbent;
• (b) meanwhile maintaining a vacuum environment of cell bottom portion to drain treated liquid solution into underneath temporary reservoir and to maintain resin/adsorbent in a semi-dry status;
• (c) intermittently collecting drained liquids in temporary reservoir and transmitting collected liquid into each assigned holding tanks in said downstream holding tanks module;
• (d) repeating repeatedly step (a) through step (c) to complete all liquid disposed at least one holding tank holding disposed in first mobile phase zone, liquid collected from cell bottom portion through which is transmitted as recycled stream to assigned holding tank in said last mobile phase zone to enhance concentration level of eluted component to save solution consumption or other usage;
• sequentially
• (e) repeating repeatedly step (a) through step (c) to complete all liquid disposed at least one holding tank holding disposed in second mobile phase zone, liquid collected from cell bottom portion through which is transmitted as recycled stream back to assigned holding tank in said second mobile phase zone to enhance concentration level of eluted component to save solution consumption or other usage;
• sequentially
• (f) repeating repeatedly step (a) through step (c) to complete all liquid disposed at least one holding tank holding disposed in third mobile phase zone, liquid collected from cell bottom portion through which is transmitted as recycled stream back to assigned holding tank in said third mobile phase zone to enhance concentration level of eluted component to save solution consumption or other usage;
• sequentially
• (g) repeating repeatedly step (a) through step (c) to complete all liquid disposed at least one holding tank holding disposed in fourth, fifth, and so forth mobile phase zone, liquid collected from cell bottom portion through which is transmitted as recycled stream back to assigned holding tank in corresponding said fourth, fifth, and so forth mobile phase zone to enhance concentration level of eluted component to save solution consumption or other usage;
• sequentially
• (h) repeating repeatedly step (a) through step (c) to complete all liquid disposed at least one holding tank holding disposed in last mobile phase zone, liquid collected from cell bottom portion through which is transmitted as recycled stream back to assigned holding tank in said first mobile phase zone to adjust and blend with components in feed solution to save solution consumption or other usage.

A generalized separation process is operated to eliminate displacement zone in chromatographic sequential operation via simultaneous respective mobile phase introducing into each of multiple cells containing particular resin/adsorbent packing material, said plurality of cells disposed in sequential order in respective zone for separating specific solute mixture containing at least one desired component from at least one other component as homogeneous liquid solution mixture in finite composition containing said solute mixture as feed solution mobile phase;

• via beginning to obtain an acceptable characteristic separation profile by carrying out a new mass transfer equilibrium contact method transmitting said liquid feed solution mobile phase contacting with a solid phase resin/adsorbent packing material, so that at least one of component contained in said liquid feed solution mixture can be adsorbed onto resin/adsorbent packing material;
• subsequently via said new mass transfer equilibrium contact method to transmit at least one of recycle liquid stream with particular solute composition dissolved in predetermined modified characteristic parameter eluent solution mixture capable of eluting specific adsorbed solute component, wherein all liquid recycled streams arranged in sequential order to contact with resin/adsorbent to separate at least one desired component migrating through various recycled streams to produce new mass transfer equilibrium contact method amid each pass of particular modified characteristic parameter eluent solution mixture to further gradually apart said at least one component from at least one of other component to obtain such acceptable characteristic separation profile;
• so that, such profile employed for said generalized separation process thereof, wherein said generalized separation process comprise following hybrid embodiments as general procedures of new mass transfer equilibrium contact method between resin/adsorbent solid phase and liquid mobile phase employed by operation of an apparatus operating via differential set-up and through single stage recycle protocol;
• thus to achieve expected simultaneous separation and concentration enhancement of at least one component isolated from at least one other component for mass production process;
• wherein
• A. beginning with determining optimal full-strength bonding capacity of resin/adsorbent with a prefixed feed solution mobile phase throughput and such solid phase amount is equivalent to mass transfer zone in chromatography to carry out same above said new mass transfer equilibrium contact method mechanism;
• wherein retaining predetermined solid phase resin/adsorbent amount distributed equally in a bundled group of predetermined quantity of columns, each column having an inlet on top side and an outlet on another side with bottom meshed filter to contain equal amount of said resin/adsorbent from being drained;
• such bundled group of columns performing like partially fluidized beds as a whole unit being named as cell hereinafter;
• wherein each column retaining equal amount of resin/adsorbent solid material as plurality of columns installed in said cell and sum amount of each disposed resin/adsorbent being corresponding to resin/adsorbent installed as mass transfer zone in chromatography to fully saturated with prefixed feed solution throughput;
• the liquid inlet of cell is from top and liquid outlet of cell is from bottom;
• said new mass transfer equilibrium contact method comprising following general procedures, wherein
• 1. intermittently delivering predetermined amounts of mobile phase liquid material through predetermined type of liquid dispenser in portion as predetermined input format dose dropping amid predetermined first time period, wherein various kind of liquid solutions including feed solution mobile phase, at least one of recycle liquid stream organized in sequential order each with particular solute composition dissolved in particular modified characteristic parameter eluent solution mixture capable of eluting specific adsorbed solute mixture component, and eluent liquid followed with pressurized inert gas in sequential contact with said resin/adsorbent to promote expected new mass transfer equilibrium contact method either promoting adsorption of dissolved components onto said resin/adsorbent or eluting adsorbed components from said resin/adsorbent;
• 2. intermittently supplying broad range pressurized inert gas to said cell top side following each delivery of said mobile phase dose means amid predetermined second time period for maintaining sufficient pressure to force prompt draining such mobile phase liquid percolate through said resin/adsorbent solid phase packing material to complete expected new mass transfer equilibrium contact method;
• 3. meanwhile maintaining a vacuum environment on the other side of said solid phase material installed in said cell amid spent time during step (2) to maintain resin/adsorbent material in a semi-dry status;
• wherein said broad range pressurized inert gas being supplied from cell top and whereas vacuum being exerted from cell bottom;
• 4. collecting most of treated mobile phase liquid material meanwhile amid spent time during step (2) from the outlet of cell bottom;
• 5. defining in sum of spent time amid step (1) through step (4) as minimal time interval;
• wherein said generalized separation process further proceed
• B. via aforesaid new mass transfer equilibrium contact method to obtain a characteristic separation profile for particular separation system, wherein feed solution mobile phase contacting with a solid phase resin/adsorbent packing material, so that at least one of component contained in said liquid feed solution mixture can be adsorbed onto resin/adsorbent packing material;
• subsequently via said new mass transfer equilibrium contact method to transmit at least one of recycle liquid stream containing particular solute composition dissolved in predetermined modified characteristic parameter eluent solution mixture capable of eluting specific adsorbed solute component, wherein all liquid recycled streams arranged in predetermined sequential order to contact with resin/adsorbent to separate at least one desired component migrating through various recycled streams to produce new mass transfer equilibrium contact method to further little by little apart said at least one component from at least one of other component amid each recycled stream contact to obtain such acceptable characteristic separation profile, further wherein various kind of mobile phase being delivered through predetermined type of liquid dispenser and combination of selected liquid input format, wherein input S-I mode is defined as that predetermined amounts of same mobile phase parametric condition is delivered within the said minimal time interval into said cell, and wherein mobile phase being delivered through predetermined type of liquid dispenser and selected liquid input format, wherein input I-I is defined as that predetermined amounts of “discrete increments” of mobile phase parametric conditions is delivered into said cell within said minimal time interval;
• C. said new mass transfer equilibrium contact method is to deliver mobile phase in format by options of said input S-I proceeded along time domain, wherein
• a. contacting said resin/adsorbent with at least one amount of feed solution mobile phase stream illustrated in step (1) through step (5) of step A and define total cumulated minimal time interval as feeding zone;
• subsequently
• b. contacting said resin/adsorbent with at least one amount of first recycle mobile phase stream in sequence, each first recycled stream comprising particular solute composition dissolved in predetermined modified characteristic parameter eluent solution mixture to reach expected new mass transfer equilibrium contact method thus at least one of component is desorbed from the resin/adsorbent in sequence illustrated in step (1) through step (5) of step A and total cumulated minimal time interval is defined as zone 1;
• subsequently;
• c. contacting said resin/adsorbent with at least one amount of second recycle mobile phase stream in sequence, each second recycled stream comprising particular solute composition dissolved in predetermined modified characteristic parameter eluent solution mixture to reach expected new mass transfer equilibrium contact method thus at least one of component is desorbed from the resin/adsorbent in sequence illustrated in step (1) through step (5) of step A and total cumulated minimal time interval is defined as zone 2;
• d. repeating step (c) of step C by adding at least one amount of third mobile phase stream if remaining components still remained been adsorbed with said resin/adsorbent, such that components can be eluted by particular solute composition dissolved in predetermined modified characteristic parameter eluent solution mixture to reach expected new mass transfer equilibrium contact method thus remaining components are desorbed from the resin/adsorbent in sequence and total cumulated minimal time interval is defined as zone 3;
• e. repeating step (d) until adsorbed component been desorbed in sequence with corresponding recycle mobile phase stream being acceptable thus total cumulated minimal time interval is defined respectively corresponding with matched particular recycle mobile phase as zone 4, 5, and so forth;
• otherwise,
• f. contacting said resin/adsorbent with at least one amount of eluent liquid then followed with pressurized inert gas to the inlet of last cell in sequence illustrated in in step (1) through step (5) of step A to flush remaining liquid and total cumulated minimal time interval is defined as last zone;
• and
• g. wherein lastly to integrate all defined zones in sequential prevail with respect to mobile phase parametric condition in time domain to conclude obtaining characteristic separation profile for particular separation system via input S-I;
• D. said new mass transfer equilibrium contact method is to deliver mobile phase in format by options of said input I-I proceeded along time domain;
• wherein
• 1) contacting said resin/adsorbent with at least one amount of feed solution mobile phase stream illustrated in step (1) through step (5) of step A and define total cumulated minimal time interval as feeding discrete zone;
• subsequently repeating this step by increasing a discrete increment of mobile phase parametric conditions until all of said at least one of feed solution mobile phase stream being adjusted with preset incremental discrete delivered in sequence and total cumulated minimal time interval is defined as multiple feeding discrete zone named in sequential order corresponding to respective delivered mobile phase parametric condition;
• subsequently
• 2) contacting said resin/adsorbent with at least one amount of first recycle mobile phase stream in sequence, each first stream comprising particular solute composition dissolved in predetermined modified characteristic parameter eluent solution mixture in discrete increment of parametric condition to reach expected new mass transfer equilibrium contact method such that at least one of component is desorbed from the resin/adsorbent in sequence illustrated in step (1) through step (5) of step A and total cumulated minimal time interval is defined as discrete zone 1;
• repeating this step by increasing a discrete increment of mobile phase parametric conditions until all of said at least one amount of first mobile phase stream being adjusted with preset incremental discrete delivered in sequence and total cumulated minimal time interval is defined as multiple discrete zone 1 named in sequential order corresponding to respective delivered mobile phase parametric condition;
• subsequently
• 3) contacting said resin/adsorbent with at least one amount of second recycle mobile phase stream in sequence, each second stream comprising particular solute composition dissolved in predetermined modified characteristic parameter eluent solution mixture of mobile phase parametric composition to reach expected new mass transfer equilibrium contact method such that at least one of component is desorbed from the resin/adsorbent in sequence illustrated in step (1) through step (5) of step A and total cumulated minimal time interval is defined as discrete zone 2;
• repeating this step by increasing a discrete increment of mobile phase parametric conditions until all of said at least one amount of second mobile phase stream being adjusted with preset incremental discrete delivered in sequence and total cumulated minimal time interval is defined as multiple discrete zone 2 named in sequential order corresponding to respective delivered mobile phase parametric condition;
• subsequently
• 4) repeating step (3) of step D adding at least one amount of third mobile phase stream if remaining components still been adsorbed with said resin/adsorbent, such that components can be eluted by particular solute composition dissolved in predetermined modified characteristic parameter eluent solution mixture with discrete increment of mobile phase parametric condition to reach expected new mass transfer equilibrium contact method such that remaining components is desorbed from the resin/adsorbent in sequence illustrated in step (1) through step (5) of step A and total cumulated minimal time interval is defined as discrete zone 3;
• repeating this step by increasing a discrete increment of mobile phase parametric conditions until all of said at least one amount of third recycle mobile phase stream being adjusted with preset incremental discrete delivered in sequence and total cumulated minimal time interval is defined as multiple discrete zone 3 named in sequential order corresponding to respective delivered mobile phase parametric condition;
• 5) repeating step (4) until adsorbed component been desorbed in sequence with corresponding recycle mobile phase stream thus total cumulated minimal time interval is defined respectively corresponding with matched particular recycle mobile phase as zone 4, 5, and so forth;
• repeating this step by increasing a discrete increment of mobile phase parametric conditions until all of said at least one amount of corresponding recycle mobile phase stream being adjusted with preset incremental discrete delivered in sequence and total cumulated minimal time interval is defined as multiple discrete zone 4, 5, and so forth named in sequential order corresponding with respective delivered recycle mobile phase parametric condition;
• 6) contacting said resin/adsorbent with at least one amount of eluent liquid then followed with pressurized inert gas to the inlet of last cell in sequence illustrated in in step (1) through step (5) of step A to flush remaining liquid and total cumulated minimal time interval is defined as last zone;
• and
• 7) wherein lastly to integrate all defined zones in sequential prevail with respect to mobile phase parametric condition in time domain to conclude obtaining characteristic separation profile for particular separation system via input I-I;
• further wherein
• E. an apparatus employed for integrating multiple modules contained in a closed loop connected in sequence yet functioning independently as a whole unit, such apparatus comprising following module, wherein
• 1) providing separation module, wherein
• (a) single cell comprising predetermined quantity of columns orderly disposed inside said cell;
• each column having an top side inlet and a bottom side outlet with meshed filter to contain equal amount of resin/adsorbent material from being drained;
• (b) a plurality of said single cell disposed inside an insulated heat media circulation jacket to maintain said plurality of cells in a selected temperature range;
• (c) each of cell top having an inlet liquid conduct as temporary transit reservoir extended outside upward of said heat media circulation jacket to receive particular liquid delivered from corresponding holding tank in downstream holding tanks module through an opened flipper, named as flipper 3 hereinafter disposed around top inside of said liquid transit storage reservoir, through liquid pipe delivering respective liquid through predetermined type of liquid dispenser down below;
• such dispenser comprising of another preferred mechanical device flipper, named as flipper 4 hereinafter disposed inside in between bottom side of said temporary transit reservoir and top side of the liquid dispenser;
• (d) each cell bottom being exposed to said vacuum environment containing a transit reservoir with its widely open top means to withdraw mist enriched inert gas via its gas exit pipe connect to a manifold to maintain said resin/adsorbent in a semi-dry status, meanwhile to affiliating liquid draining via disposed funneled shape liquid conduct through;
• (e) another opened mechanical flipper, names as flipper 65 hereinafter disposed inside bottom of said funnel conduct, into underneath temporary liquid reservoir;
• having a gas pipe connected with same manifold next to said mist enriched inert gas exit pipe means for supplying broad range pressurized inert gas via this manifold to shut off said flipper 65 soon said vacuum environment being shut off, so that pushing entire drained liquid into following module via a preferred pressurize activated check valve disposed around top inside of said bottom liquid conduct, wherein said inert gas manifold being disposed underneath said vacuum environment and being extended outside downward of said insulated heat media circulation jacket;
• 2) providing downstream holding tanks module, wherein
• (a) having a plurality of holding tank disposed in an organized order setting inside an insulated heat media circulation jacket to maintain whole plurality of holding tanks in a selected temperature range and each tank having an inlet liquid conduct extended outside upward of the insulated heat media circulation jacket;
• (b) each of said whole plurality holding tanks comprising an outlet liquid conduct installed with said preferred pressure activated check valve extended outside downward of said jacket means for discharging stored liquid via each means of liquid distribution;
• (c) having a liquid level sensor installed inside each of said whole plurality holding tanks to monitor predetermined liquid level of stored liquid within for following;
• (i) means such level sensor is to control delivering sufficient volume amount of particular solution via liquid conduct disposed on top of such holding tank to maintaining a predetermined liquid level setting in respective holding tank and holding received respective liquid;
• (ii) means for at least one isolated product into respective assigned storage tank;
• (iii) means for discharging components in particular solution mixture as by product into respective assigned storage tank;
• and
• (iv) means for transmitting in part of recycled stream of particular solute composition dissolved in predetermined modified characteristic parameter eluent solution mixture stored in assigned holding tank in predetermined volume recycling back into each assigned cell top in aforesaid separation module;
• further wherein;
• 3) providing inert gas supply module means for mobile phase liquid streams transmitting mechanism for close loop routing supplying broad range pressurized inert gas inasmuch as incorporating with liquid fluid transmitting between said separation module and downstream holding tanks module, wherein such module contain closed vacuum environment loop, upstream broad range inert gas supplying loop, and downstream broad range inert gas supplying loop;
• (a) proceeding closed vacuum environment loop comprising following procedures,
• (i) cell bottom been exposed to said vacuum environment to affiliate dropped dose liquid draining, meanwhile to extracting mobile phase liquid enriched mist inert gas to maintain said resin/adsorbent in semi-dry status through means extracting mist enriched inert gas to dry inert gas via driving force exerted from central vacuum pump through gas pipe via a selected mist separator to recover such mist from enriched inert gas;
• (ii) meanwhile to create a heterogeneous contact as following dropped dose of liquid promptly sipping through stationary resin/adsorbent particles to meet criterion of said new mass transfer equilibrium contact method;
• (iii) whole time, dry inert gas exiting mist separator being combined with pressurized dry air and deployed through an inert gas generator to obtain fresh dry inert gas and stored in a steel tank vessel maintaining preferred broad range of pressure level inert gas ready deploying back to following modules;
• (b) proceeding upstream broad range inert gas supplying loop means for cell top region in separation module wherein comprising following procedures,
• (i) via each volumetric pump simultaneously transmitting predetermined liquid volume amount from assigned holding tank of said downstream holding tanks module via supplying of broad range pressurized inert gas through gas pipe connected to close said flipper 4 disposed around each bottom of said temporary transit reservoir to hold transmitted liquid and thus to freely passing through opened said flipper 3 disposed around each cell top liquid conduct into each said cell top temporary transit reservoir located at top of said separation module;
• soon entire transmitted liquid amount being completed;
• subsequently
• (ii) via means of alternatively and simultaneously supplying of broad range pressurized inert gas through gas pipe connected in between said flipper 3 and flipper 4 of said temporary transit reservoir and another gas pipe disposed next to said temporary transit reservoir to intermittently dose dropping in parts of received liquid out of temporary transit reservoir resulting as predetermined said liquid input format to promptly wet top portion and promptly sipping through resin/adsorbent bed to carry out expected new mass transfer equilibrium contact method;
• (c) proceeding downstream broad range inert gas supplying loop means for all cells bottom region in separation module and downstream holding tanks module wherein comprising following procedures
• (i) during duration of exerted vacuum environment to continuously drain dropped doses of liquid into underneath liquid reservoir and said preferred pressure activated check valve holding drained liquid within;
• soon as liquid draining of entire transmitted liquid been completed;
• subsequently;
• (ii) shutting off vacuum environment, supplying low range pressurized inert gas routing via its pipe disposed next to said vacuum exit pipe in same manifold to close said flipper 65 to allow entire collected liquid freely pushing through opened said pressure activated check valve through liquid line connected to assigned holding tank in said downstream holding tanks module;
• F. as illustrated wherein differential set-up protocols employed via illustrated in step (1) through step (5) of step A for general procedures of new mass transfer equilibrium contact method onto aforesaid predetermined cell construction of step A to carry out by predetermined combination of selected mobile phase between in-put S-I and in-put I-I format to obtain a characteristic separation profile for particular separation system related between selected resin/adsorbent and various kinds of mobile phase liquid material onto aforesaid apparatus to separate at least one desired component from feed solution mobile phase, following is said differential set-up protocol employed onto said apparatus, wherein
• 1. determining optimal full-strength bonding capacity of said resin/adsorbent with a prefixed feed throughput and filling such resin/adsorbent amount into a said cell contain multiple column disposed within;
• 2. sequentially breaking down characteristic elution profile partial time required to spend for respective predetermined mobile phase solution, this including feed solution mobile phase, at least one of recycle liquid stream organized in sequential order each with particular solute composition dissolved in particular modified characteristic parameter eluent solution mixture capable of eluting specific adsorbed solute mixture component, and eluent liquid followed with pressurized inert gas;
• 3. divide each said partial time by said minimal time interval to obtain the range cumulated minimal time interval and sum as major minimal time interval for multiple cells disposed in respective zone including feeding zone and other zones following same major time interval of most time-consuming zone;
• then
• 4. divide total input volume amount of such liquid by the number of cells to obtain the partial volume required for each cell in respective zone;
• then, further divides such said partial volume by a preselected number to further differentiate for multiple liquid doses to reflect general procedures of said new mass transfer equilibrium contact method sipping through resin/adsorbent in each cell;
• in addition
• 5. divides said resin/adsorbent amount in step 1, which derived from complete saturation with feed solution throughput, by a preselected number that corresponds to differential resin/adsorbent amount disposed in at least one column bundled in a single cell disposed in each zone to simultaneously receive the partial volume of such liquid for each cell in said group of cells;
• 6. sequentially allocate all cells with respective mobile phase solution as the range of respective zone and allocate all zones into an endless format;
• wherein integrating all zones orderly arranged in sequential format representing a complete elution separation cycle via transmitting all kind of mobile phase with finite parameter solution into underneath stationary separation module is to simulate all cells in horizon moving direction whereas mobile phase is simultaneously traveling in vertical direction via predetermined combination of selected mobile phase between in-put S-I between I-I format, wherein
• (a) including setting up at least one zone between selection of at least one of feed solution mobile phase stream and multiple discrete feed solution mobile phase stream as feeding zone;
• (b) including setting up at least one zone between selection of at least one of first recycle mobile phase stream and multiple first discrete recycle mobile phase stream as zone 1;
• (c) including setting up at least one zone between selection of at least one of second recycle mobile phase stream and multiple second recycle discrete mobile phase stream as zone 2;
• (d) including setting up at least one zone between selection of at least one of third mobile phase stream and multiple third recycle discrete mobile phase stream as zone 3;
• (e) further expand including setting up in sequence at least one of additional zone between selection of fourth, fifth, and so forth mobile phase stream and multiple fourth, fifth, and so forth recycle discrete mobile phase streams as additional name as zone 4, 5, and so forth;
• (f) including setting up at least one zone corresponding to at least one of eluent liquid followed with pressurized inert gas stream as last zone;
• (g) and a predetermined plurality of alternative recycle mobile phase streams back to predetermined cell top temporary transit reservoir to further enhance concentration of eluted component dissolved in particular modified characteristic parameter eluent solution mixture capable of further eluting specific adsorbed solute mixture component and save consumption of respective eluent solution mixture;
• (h) thus, integrating all combination of aforesaid zones in sequential order representing such obtained characteristic separation profile being for eliminating chromatography displacement zone and simultaneous separating at least one desired component from at least one other component amid each spent of minimal time interval;
• G. further arranging such differential set-up protocols onto said apparatus via said new mass transfer equilibrium contact method along with single stage recycle protocol employed onto said apparatus to achieve separation operation containing following:
• 1) providing said downstream holding tanks module containing a plurality of holding tanks disposed in approximate order as corresponding each cell disposed in said separation module to hold drained corresponding liquid transmitted from and transmitted to assigned cell and other destination;
• such plurality holding tanks setting inside an insulated heat media circulation jacket to maintain whole plurality in a selected temperature range;
• wherein
• a. at least one holding tank arranged in sequence assigned for receiving in corresponding with feed solution mobile phase containing at least one desired component from at least one other component as homogeneous liquid solution mixture in finite composition transmitted from its source into assigned top portion of cell top temporary transit reservoir in contact with resin/adsorbent solid phase packing material to attain new mass transfer equilibrium contact method as treated liquid to promote adsorption of at least one solute component collected from particular temporary holding tank disposed in cell bottom portion of said separation module as liquid holding tank disposed in feeding zone;
• b. at least one holding tank arranged in sequence assigned for receiving in corresponding with particular recycled stream containing solute composition dissolved in particular modified characteristic parameter eluent solution mixture transmitted from said zone 1 into assigned top portion of cell top temporary transit reservoir in contact with resin/adsorbent solid phase packing material to attain new mass transfer equilibrium contact method as treated liquid to promote eluting specific adsorbed solute mixture component collected from particular temporary holding tank disposed in cell bottom portion of said separation module as liquid holding tank disposed in zone 1;
• c. at least one holding tank arranged in sequence assigned for receiving in corresponding with particular recycled stream containing solute composition dissolved in particular modified characteristic parameter eluent solution mixture transmitted from said zone 2 into assigned top portion of cell top temporary transit reservoir in contact with resin/adsorbent solid phase packing material to attain mass transfer equilibrium as treated liquid to promote eluting specific adsorbed solute mixture component collected from particular temporary holding tank disposed in cell bottom portion of said separation module as liquid holding tank disposed in zone 2;
• d. at least one holding tank arranged in sequence assigned for receiving in corresponding with particular recycled stream containing solute composition dissolved in particular modified characteristic parameter eluent solution mixture transmitted from said zone 3 into assigned top portion of cell top temporary transit reservoir in contact with resin/adsorbent solid phase packing material to attain new mass transfer equilibrium contact method as treated liquid to promote eluting specific adsorbed solute mixture component collected from particular temporary holding tank disposed in cell bottom portion of said separation module as liquid holding tank disposed in zone 3;
• e. further expand at least one holding tank arranged in sequence assigned for receiving in corresponding with particular recycled stream containing solute composition dissolved in particular modified characteristic parameter eluent solution mixture transmitted from each said zone 4, 5, and so forth into assigned top portion of cell top temporary transit reservoir in contact with resin/adsorbent solid phase packing material to attain new mass transfer equilibrium contact method as respective treated liquid to promote eluting specific adsorbed solute mixture component collected from particular temporary holding tank disposed in cell bottom portion of said separation module as corresponding liquid holding tank disposed in sequence with corresponding zone 4, 5, and so forth;
• f. at least one holding tank arranged in sequence assigned for receiving in corresponding with eluent liquid transmitted from its source into assigned top portion of cell top temporary transit reservoir in contact with resin/adsorbent solid phase packing material to attain mass transfer equilibrium as treated liquid to promote eluting all remaining adsorbed solute mixture component collected from particular temporary holding tank disposed in cell bottom portion of said separation module as liquid holding tank disposed in last zone;
• g. adding one holding tank arranged in sequence after last zone assigned for receiving in corresponding with pressurized inert gas from its source into assigned top portion of cell top temporary transit reservoir to flush remaining liquid in resin/adsorbent solid phase packing material to maintain semi-dry status of new mass transfer equilibrium contact method collected from particular temporary holding tank disposed in cell bottom portion of said separation module as last holding tank disposed in sequence after last zone;
• further
• h. assign according to step (a) through step (g) at least one product holding tank for respectively holding said one particular enriched component product and at least one by-product holding tank for respectively holding at least one other enriched component by-product;
• further preparing each recycle liquid stream according to obtained characteristic elution profile with finite solute composition dissolved in particular modified characteristic parameter eluent solution mixture capable of eluting specific adsorbed solute mixture component from respective holding tank organized in sequential order as said zone 1, 2, 3, 4, 5, and so forth, and “last zone” to hold eluent liquid transmitted from its source, followed with last holding tank to hold remaining liquid flushed by pressurized inert gas after last zone;
• thus integrating whole spectrum of holding tanks representing various kind of liquids of produced characteristic elution profile organized in sequential order disposed in each holding tanks of said downstream holding tanks module;
• and hence
• 2) proceeding said single stage recycle protocol incorporating said downstream holding tanks module with providing said separation module to achieve separation operation;
• wherein separation module comprising a plurality of each cell to retain predetermined solid phase resin/adsorbent amount as sum to fully saturated with prefixed feed solution throughput as mass transfer zone in chromatography and to distribute equal sub-portion amount in a plurality of each column having an inlet on top side and an outlet on bottom meshed filter to contain disposed resin/adsorbent from being drained;
• said such plurality of cells being arranged in approximate order as said downstream holding tanks module disposed in an insulated heat media circulation jacket to maintain in a selected temperature range;
• via general procedures of said new mass transfer equilibrium contact method, each assigned cell simultaneously receive predetermined volume amount of respective liquid stream via said mobile phase liquid streams transmitting mechanism of aforesaid various kind of mobile phase liquid stream from respective source in a format of predetermined volume in sequential dose drop into said separation module to produce expected new mass transfer equilibrium contact method, wherein including liquid from said feeding zone, zone 1, 2, 3, 4, 5, and so forth corresponding to feed solution mobile phase, each recycle liquid stream from respective holding tank organized in sequential order with particular solute composition dissolved in particular modified characteristic parameter eluent solution mixture capable of eluting specific adsorbed solute mixture component, and last zone of eluent liquid followed with pressurized inert gas;
• all liquid streams being simultaneously delivered into each assigned cell top temporary transit reservoir via said liquid streams transmitting mechanism in intermittent and simultaneous contact with resin/adsorbent, wherein after each drop dose of simultaneous liquid delivery following repeated procedures via repeated said new mass transfer equilibrium contact method proceeding through each assigned cell disposed in said separation module, wherein
• (a) intermittent and simultaneous delivering amid first time period through means of alternated supplying between two separated broad range of pressurized inert gas routings from respective cell top portion to force draining of dose dropped liquid promptly sipping through said resin/adsorbent to complete said new mass transfer equilibrium contact method between two phases to promote adsorption of components onto said resin/adsorbent or eluting adsorbed component from said resin/adsorbent;
• (b) meanwhile maintaining a vacuum environment of cell bottom portion to drain treated liquid solution into underneath temporary reservoir thus to maintain resin/adsorbent in a semi-dry status;
• (c) intermittently amid second time period collecting treated liquid in temporary reservoir;
• (d) repeating repeatedly step (a) through step (c) to complete all simultaneous liquid delivering from its source including feed solution, a plurality of recycle liquid stream in zone 1, 2, 3, 4, 5, and so forth along with last zone of each predetermined volume liquid amount into each assigned temporary transit reservoir of said top portion in separation module, so that, at least one desired component migrating through various recycled streams to produce new mass transfer equilibrium contact method amid each passage of liquid dose drop of particular modified characteristic parameter eluent solution mixture to further gradually apart said at least one component from at least one of other component to obtain such separation result of said characteristic separation profile amid each spent of said major time interval;
• further transmitting treated liquid via mobile phase liquid streams transmitting mechanism back to each assigned holding tanks in said downstream holding tanks module, including at least one product holding tank for respectively holding said one particular enriched component product, at least one by-product holding tank for respectively holding at least one other enriched component by-product, and a plurality of recycled mixtures orderly contained in each of corresponding holding tank, so that, each tank contains one particular recycled mixture that has a specific composition of said components dissolved in drained solution and eluent collected from bottom portion of said separation module for repeated recycling back to said respective assigned temporary transit reservoir in said top portion of separation module.