Adas comprising type 1 pili
AssigneeFlagship Pioneering Innovations VI, LLC
InventorsDavid Barry KOLESKY, Caitlin Nicole SPAULDING, Troy Patrick HUBBARD, Charles Martin FOX, Kimberly A. HOMAN
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Abstract
Provided herein are achromosomal dynamic active systems comprising a Type 1 pilus (TIP) and methods of making and using the same.
Claims62
A preparation comprising a plurality of achromosomal dynamic active systems (ADAS) derived from parent bacterial cells genetically engineered to constitutively express a type 1 pilus (T1P), wherein the plurality of ADAS binds a target cell via the T1P.
An ADAS derived from a parent bacterial cell genetically engineered to constitutively express a T1P, wherein the ADAS is produced by a process comprising the steps of:
- (a) providing a parent bacterial cell that has been modified to constitutively express the components of a T1P;
- and
- (b) producing an ADAS from the parent bacterial cell, wherein the ADAS comprises the T1P.
An ADAS derived from a parent bacterial cell genetically engineered to constitutively express a T1P, wherein the ADAS is produced by a process comprising the steps of:
- (a) providing a parent bacterial cell that has been modified to express the components of a native T1P at a level that is at least 1.5-fold greater than a level observed in an unmodified parent bacterial cell;
- and
- (b) producing an ADAS from the parent bacterial cell, wherein the ADAS comprises the T1P that is native to the parent bacterial cell.
An ADAS derived from a parent bacterial cell genetically engineered to constitutively express a T1P, wherein the ADAS is produced by a process comprising the steps of:
- (a) providing a parent bacterial cell that has been modified to express the components of a heterologous T1P;
- and
- (b) producing an ADAS from the parent bacterial cell, wherein the ADAS comprises the T1P that is heterologous to the parent bacterial cell.
A genetically engineered bacterium that constitutively expresses the components of a T1P, wherein the engineered bacterium comprises the T1P at a level which is at least 1.5-fold greater compared to the level of T1P comprised by a non-engineered bacterium.
A genetically engineered bacterium that constitutively expresses the components of a T1P, wherein the bacterium has been modified to produce ADAS.
A method for producing an ADAS, the method comprising producing an ADAS from a bacterium genetically engineered to constitutively expresses the components of a T1P.
Dependencies are inferred from the claim text.