Methods of assaying proteins
OwnerParag MALLICKinventor
Life of this patent
Estimating the term…
Abstract
Methods and systems for identifying a protein within a sample are provided herein. A panel of antibodies are acquired, none of which are specific for a single protein or family of proteins. Additionally, the binding properties of the antibodies in the panel are determined. Further, the protein is iteratively exposed to a panel of antibodies. Additionally, a set of antibodies which bind the protein are determined. The identity of the protein is determined using one or more deconvolution methods based on the known binding properties of the antibodies to match the set of antibodies to a sequence of a protein.
Claims20
A method of characterizing polypeptides, comprising:
- providing a plurality of polypeptides attached to a surface of a substrate, with individual polypeptide molecules being attached at individually addressable locations on the substrate;
- contacting the polypeptides on the array with at least one hundred different affinity reagents, where each of the at least one hundred different affinity reagents comprises a binding affinity for a different epitope that is present in more than one of the plurality of polypeptides;
- separately detecting binding of each of the at least one hundred different affinity reagents to the individual polypeptide molecules on the substrate;
- and
- characterizing each of the plurality of polypeptide molecules based upon the different affinity reagents that bind to each of the polypeptide molecules.
Dependencies are inferred from the claim text.