# US 2025/0367243 A1 · Bacillus isolate compositions and methods of using and producing the same > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2025/0367243 A1 - Document ID: US20250367243A1 - Status: Published application - Kind: A1 - Application number: 19/257,302 - Filed: 2025-07-01 - Published: 2025-12-04 - Earliest claimed: 2019-06-21 - Assignees: Board of Trustees of the University of Arkansas - Applicants: The Board of Trustees of the University of Arkansas - Inventors: Billy M. Hargis; Lucas Elzie Graham; Kyle Dean Teague; Juan David Latorre; Samuel J. Rochell - Web page: https://patentgrep.com/patent/US20250367243A1 ## CPC - A61K 35/742: Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes - A23L 33/135: Bacteria or derivatives thereof, e.g. probiotics - A61K 35/74: Bacteria (therapeutic use of a bacterial protein A61K38/00) - C12N 1/20: Bacteria; Culture media therefor - C12N 1/205: Bacterial isolates - C12R 2001/07: Bacillus - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B) - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS ## Abstract The present invention provides Bacillus isolates, as well as probiotic and animal feed compositions comprising said isolates, which may be included in the diet of poultry to improve growth performance with reduced energy diets. Methods of using these compositions to improve nutritional uptake or to reduce the incidence of footpad dermatitis in poultry are also provided. ## Claims ### Claim 1 An animal feed composition comprising a plant-based food source and at least one Bacillus isolate selected from: a) a Bacillus isolate designated as “Isolate 46” and deposited at the Agricultural Research Service Culture Collection (NRRL) under accession number B-67957; and b) a Bacillus isolate designated as “Isolate 40” and deposited at the NRRL under accession number B-67956. ### Claim 2 (depends on 1) The animal feed composition of claim 1, wherein the at least one Bacillus isolate is in spore form. ### Claim 3 (depends on 2) The animal feed composition of claim 2, wherein the animal feed composition comprises between about 104-1012 spores of the at least one Bacillus isolate per gram of total animal feed composition. ### Claim 4 (depends on 1) The animal feed composition of claim 1, wherein the plant-based food source comprises an anti-nutritional factor. ### Claim 5 (depends on 4) The animal feed composition of claim 4, wherein the anti-nutritional factor is selected from the group consisting of raffinose, stachyose, verbascose, cellulose, hemicellulose, galactomannan, xylan, arabinoxylan, xylose, and phytate. ### Claim 6 (depends on 1) The animal feed composition of claim 1, wherein the plant-based food source is or is derived from soybean, canola, sunflower, palm kernel, copra, linseed, peanut, sesame seed, corn, wheat, sorghum, barley, rye, triticale, or oats. ### Claim 7 (depends on 1) The animal feed composition of claim 1, wherein the animal feed composition is a low energy diet (LED) that has 1-10% lower energy content as compared to a standard animal diet as measured in kcal/kg2. ### Claim 8 (depends on 1) The animal feed composition of claim 1, wherein the animal feed composition comprises both Isolate 46 and Isolate 40. ### Claim 9 (depends on 1) A method for improving nutritional uptake in an animal, the method comprising administering to the animal an effective amount of the composition of claim 1. ### Claim 10 (depends on 9) The method of claim 9, wherein the improved nutritional uptake in the animal is demonstrated by improved body weight gain, improved cumulative feed conversion ratio, or improved carcass characteristics. ### Claim 11 (depends on 1) A method for producing the animal feed composition of claim 1, the method comprising introducing the at least one Bacillus isolate into a plant-based food source to form the animal feed composition. ### Claim 12 (depends on 11) The method of claim 11, further comprising pelleting the animal feed composition. ### Claim 13 (depends on 11) The method of claim 11, wherein the at least one Bacillus isolate is in spore form. ### Claim 14 (depends on 13) The method of claim 13, wherein the animal feed composition comprises between about 104-1012 spores of the at least one Bacillus isolate per gram of total animal feed composition. ### Claim 15 (depends on 11) The method of claim 11, wherein the plant-based food source comprises an anti-nutritional factor. ### Claim 16 (depends on 15) The method of claim 15, wherein the anti-nutritional factor is selected from the group consisting of raffinose, stachyose, verbascose, cellulose, hemicellulose, galactomannan, xylan, arabinoxylan, xylose, and phytate. ### Claim 17 (depends on 11) The method of claim 11, wherein the plant-based food source is or is derived from soybean, canola, sunflower, palm kernel, copra, linseed, peanut, sesame seed, corn, wheat, sorghum, barley, rye, triticale, or oats. ### Claim 18 (depends on 11) The method of claim 11, wherein the plant-based food source is fermented with the at least one Bacillus isolate prior to packaging. ### Claim 19 (depends on 11) The method of claim 11, wherein the animal feed composition is a low energy diet (LED) that has 1-10% lower energy content as compared to a standard animal diet as measured in kcal/kg2. ### Claim 20 (depends on 11) The method of claim 11, wherein the animal feed composition comprises both Isolate 46 and Isolate 40.