A method of preparing a composition for regenerating cartilage using lyophilization hyaline cartilage powder, comprising:
- A) preparing hyaline cartilage;
- B) preparing lyophilization hyaline cartilage powder by freeze-drying and crushing the hyaline cartilage;
- C) preparing an adipose tissue extract from an autologous adipose tissue;
- and
- D) preparing a composition for regenerating cartilage which includes the lyophilization hyaline cartilage powder and the adipose tissue extract,
- wherein Step C) comprises C1) removing fibers in the autologous adipose tissue using a first filter having a pore diameter of 2 to 3 mm;
- and C2) obtaining an adipose tissue extract by sequentially filtering a fiber-free autologous adipose tissue through a second filter having a pore diameter of 450 to 550 μm and a third filter having a pore diameter of 150 to 250 μm, and
- wherein the concentration of the lyophilization hyaline cartilage powder is 5 mg to 70 mg per 1 mL of the composition.