Population of transfected immune cells and method for their production

Abstract

An in-vitro or ex-vivo method for transiently modifying immune cells in a closed processing system is disclosed. The steps include: (i) providing immune cells from a biological liquid and/or from a resected tumor from a patient; (ii) purifying the immune cells; (iii) transfecting the purified immune cells with an inhibitory nucleic acid of a immunosuppressive regulator of the immune cells or with a nucleic acid of an immune enhancing factor; (iv) rebuffering the transfected immune cells into a physiological solution; (v) transferring the transfected immune cells into a container; wherein the transitions between steps (i) to (v) are in a closed container system; and a therapy using the transfected immune cells.

Dependencies are inferred from the claim text.