Compositions and Methods for Producing High-Protein Pea Plants
OwnerJanice Kofsky, David Larson +1inventors
InventorsJanice Kofsky, David Larson, Herbert Wolfgang Goettel
Life of this patent
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Abstract
Provided herein are methods for producing pea plants having high protein using marker-assisted selection. The disclosure further provides methods for introgressing one or more loci comprising at least a high-protein allele linked to the high-protein QTL, thus producing high-protein pea plants.
Claims29
A method of producing a population of high-protein pea plants or seeds, said method comprising:
- a) genotyping a first population of pea plants or seeds for the presence of at least one high-protein molecular marker that is within 20 centimorgans of one or more high-protein Quantitative Trait Locus (QTLs) selected from the group consisting of Ps03_531239107, Ps05_49389403, Ps01_20222535, Ps01_22514126, Ps01_55991509, Ps01_78756169, Ps01_87632539, Ps01_88206114, Ps01_95579585, Ps01_113369982, Ps01_113369984, Ps01_120406755, Ps01_160458316, Ps01_264925535, Ps01_279286967, Ps01_280789385, Ps01_300614888, Ps01_324252121, Ps01_349096914, Ps01_367706416, Ps01_380906255, Ps01_436651445, Ps01_440892085, Ps02_17543117, Ps02_64161520, Ps02_149117835, Ps02_162193050, Ps02_249953551, Ps02_282186543, Ps02_293278647, Ps02_296256342, Ps02_298578096, Ps02_313767424, Ps02_389051201, Ps02_432513197, Ps02_440456554, Ps03_158264810, Ps03_205819517, Ps03_206829164, Ps03_238101773, Ps03_241025997, Ps03_481796573, Ps03_483314788, Ps03_507346266, Ps03_511404191, Ps03_513771826, Ps03_531014546, Ps03_531232613, Ps04_9648139, Ps04_26115694, Ps04_106176050, Ps04_119030031, Ps04_126746363, Ps04_133748675, Ps04_140768543, Ps04_196413843, Ps04_198084088, Ps04_198169869, Ps04_256098157, Ps04_263312773, Ps04_284358817, Ps04_327258970, Ps04_347955117, Ps04_374415380, Ps04_378090615, Ps04_386293806, Ps04_434414625, Ps04_445153308, Ps04_457675265, Ps04_463538432, Ps04_464099084, Ps04_467088335, Ps05_5262178, Ps05_17115394, Ps05_23320549, Ps05_48702172, Ps05_51336818, Ps05_53552642, Ps05_54722636, Ps05_134772954, Ps05_139126831, Ps05_173144250, Ps05_175640373, Ps05_217776534, Ps05_265627777, Ps05_268032915, Ps05_277838646, Ps05_284520856, Ps05_289702502, Ps05_290623435, Ps05_320108884, Ps05_337541850, Ps05_338232797, Ps05_352490739, Ps05_358014672, Ps05_409869744, Ps05_456631333, Ps05_500234888, Ps05_534247077, Ps05_543517276, Ps05_550603121, Ps05_551582581, Ps05_556990553, Ps05_564305756, Ps05_568744565, Ps05_576520275, Ps05_591946858, Ps05_596172019, Ps06_1859845, Ps06_32259152, Ps06_71058460, Ps06_75832558, Ps06_79052113, Ps06_91302660, Ps06_97595572, Ps06_108179595, Ps06_137271101, Ps06_261243645, Ps06_375201129, Ps06_383667570, Ps06_402503684, Ps07_46743665, Ps07_50335973, Ps07_55350864, Ps07_57031312, Ps07_58281807, Ps07_84885129, Ps07_89781713, Ps07_112377551, Ps07_131261098, Ps07_155895151, Ps07_173321635, Ps07_231299734, Ps07_235684752, Ps07_238767894, Ps07_241735133, Ps07_274069066, Ps07_314769485, Ps07_327087818, Ps07_337883272, Ps07_466233654, Ps07_466821729, and Ps07_482615897;
- b) selecting from the first population one or more pea plants or seeds comprising one or more high-protein alleles having the at least one high-protein molecular marker;
- and
- c) producing a second population of progeny pea plants or seeds from the selected one or more pea plants or plants grown from the selected seeds, wherein the second population of progeny pea plants or seeds comprises the one or more high-protein alleles having the at least one high-protein molecular marker,
- and wherein the second population of progeny pea plants or seeds are high-protein pea plants or seeds, thereby producing a population of high-protein pea plants or seeds.
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A method of introgressing a high-protein QTL, the method comprising:
- (a) crossing a first pea plant comprising a high-protein QTL with a second pea plant of a different genotype to produce one or more progeny plants or seeds;
- and
- (b) selecting a progeny plant or seed comprising one or more high-protein alleles of a polymorphic locus linked to the high-protein QTL,
- wherein the polymorphic locus is a chromosomal segment comprising any marker within genomic regions of a Pisum sativum genome corresponding to genomic regions 421,829,254-437,541,609 of chromosome 3, 1-54716217 of chromosome 5, 20877277-371072249 of chromosome 1LG6, 10842575-426699364 of chromosome 2LG1, 104891818-425968089 of chromosome 3LG5, 5972665-445125850 of chromosome 4LG4, 362278-547326524 of chromosome 5LG3, 1621846-438943399 of chromosome 6LG2, 8316015-481276628 of chromosome 7LG7, scaffold 02116, scaffold 04655, scaffold 00066, scaffold 03789, scaffold 02021, scaffold 00644, scaffold 00254, scaffold 02127, scaffold 00706, super-scaffold 888, scaffold 00462, scaffold 02449, scaffold 02833, scaffold 00839, scaffold 05469,, scaffold 06512, scaffold 02959 scaffold 00840, or scaffold 01757 of the Cameor v1a reference genome.
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A nucleic acid molecule for detecting a high-protein molecular marker in a Pisum sativum genome, wherein the nucleic acid molecule comprises at least 15 nucleotides that include or are immediately adjacent to the marker, wherein the nucleic acid molecule is at least 90 percent identical to a sequence of the same number of consecutive nucleotides in either strand of DNA that include or are immediately adjacent to the marker.
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Dependencies are inferred from the claim text.