Applications of o2-insensitive formate dehydrogenase
AssigneeUniversity of Maryland, Baltimore
InventorsC. S. RAMAN, Joel E. GRAHAM
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Abstract
Disclosed are methods and apparatuses utilizing an O2-insensitive FDH2 from the sulfate-reducing bacterium (SRB) Desulfovibrio vulgaris Hildenborough (DvH). The O2-insensitive FDH2 may be applied to a biofuel cell for generating electricity and generating hydrogen peroxide. The biofuel cell can also be applied to wearable or implantable devices as a power source. The O2-insensitive FDH2 can also be used in other applications not applying a fuel cell, such as hydrogen peroxide generation, a formate testing kit, or carbon capture applications.
Claims18
A biofuel cell, comprising:
- a chamber,
- a bioanode comprising formate dehydrogenase (DvH-FDH2) derived from Desulfovibrio vulgaris Hildenborough adsorbed thereon,
- a biocathode comprising laccase, bilirubin oxidase, or cytochrome cbd oxidase (CydCBD) adsorbed thereon, and
- an electrolyte comprising a buffer including formate,
- wherein the bioanode and the biocathode are electrically connected to form an electric circuit, and
- wherein the DvH-FDH2 is O2-insensitive.
A kit for generating hydrogen peroxide, comprising:
- a matrix having formate dehydrogenase (DvH-FDH2) derived from Desulfovibrio vulgaris Hildenborough immobilized thereon, the DvH-FDH2 being O2-insensitive, and
- a source of oxygenated formate, and
- an apparatus which flows oxygenated formate to the matrix.
A kit for detecting formate, comprising:
- a reaction buffer,
- a formate standard, and
- formate dehydrogenase (DvH-FDH2) derived from Desulfovibrio vulgaris Hildenborough, the DvH-FDH2 being O2-insensitive, and
- a mediator dye.
A device for converting carbon dioxide in air to formate, comprising:
- a chamber,
- an electrolyte comprising a buffer including formate,
- a bioanode comprising an enzyme adsorbed thereon, the enzyme being capable of injecting electrons into the electrolyte,
- a biocathode comprising formate dehydrogenase (DvH-FDH2) derived from Desulfovibrio vulgaris Hildenborough adsorbed thereon, and
- an air supply which injects air containing carbon dioxide into the electrolyte,
- wherein the bioanode and biocathode are electrically connected to an electric circuit, and
- wherein the DvH-FDH2 is O2-insensitive.
Dependencies are inferred from the claim text.