Urinary branched-chain amino acids (ubcaas) as insulin resistance biomarkers
AssigneesSkillcell, Centre National De La Recherche Scientifique, Universite De Montpellier, Centre Hospitalier Universitaire De Montpellier
InventorsFrancisco SANTOS SCHNEIDER, Franck MOLINA, Eric RENARD
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Abstract
The present invention is directed to method of determining whether a subject is at risk of developing insulin resistance, particularly for advance alert of T2D and or CVD onset in obese and non-obese subject, by detecting the branched-chain amino acids (BCAAs) present in an urine sample (uBCAAs) of the subjects. The present invention also relates to a method for determining the need of a dietary/nutritional supplement for a subject involving said uBCAAs biomarkers. Finally, the invention is directed to kit comprising the biochemical network allowing the uBCAAs detection and process for the preparation of said biochemical networks as diagnostic biomarker.
Claims24
An in vitro method of determining whether a subject is at risk of being developing or to develop insulin resistance/future Type 2 diabetes (T2D) and/or cardiovascular diseases CVD/for advance alert of T2D and/or CVD onset/for the detection of insulin-resistant subjects at risk of early T2D and/or CVD onset/for the diagnostic and monitoring of insulin-resistance individual from an insulin-sensitive one, the method comprising:
- a) from an urine sample obtained from the subject, measuring/determining the concentration of branched-chain amino acids (BCAAs) present in said urine sample (uBCCAs) by an enzymatic reaction network by gathering the urine sample with a solution containing at least Leucine dehydrogenase (LeuDH) enzyme;
- and
- determining whether the subject is at said risk, wherein a uBCCAs concentration superior or egal to a cut-off (threshold)), is indicative that the subject is at risk of developing or develop insulin resistance/future T2D and/or CVD/can distinguish insulin-resistant individuals from insulin-sensitive ones/for advance alert of T2D and/or CVD onset/for the detection of insulin-resistant subjects at risk of early T2D and/or CVD onset.
A method or a process of determining the need or deficiency of a dietary/nutritional supplement for a subject or to analyze the nutritional needs or deficiency of a subject by considering a combination of various health and performance factors (health profile);
- comprising:
- a) from an urine sample obtained from the subject, measuring/determining at least the concentration of branched-chain amino acids (BCAAs) present in said urine sample (uBCCAs) by an enzymatic reaction network by gathering the urine sample with a solution containing at least Leucine dehydrogenase (LeuDH) enzyme;
- b) comparing the result obtained in step a) to the concentration of uBCAAs present in a subject exhibiting a normal health profile and/or control health profile(s) known to require a personalized regimen;
- c) determining whether the subject is in need or deficiency of nutritional/dietary supplement(s) and/or requires a personalized regimen or therapy wherein the presence of a uBCCAs concentration superior or egal to a cut-off (threshold)), is indicative that the subject is in need or deficiency of dietary/nutritional supplement(s) and/or personalized regimen or therapy;
- d) optionally, preparing a personalized regimen for the subject, the regimen including a customized nutritional formula and/or synergistic physical program and/or personalized therapy;
- and
- administering the regimen to the subject and/or performing the synergistic physical program and/or the personalized therapy over a period of time;
- e) optionally, further comprising re-analyzing the subject's needs or deficiencies and, if necessary, adjusting the regimen or the therapy.
A kit comprising:
- Ascorbate oxidase and LeuDH, preferably in solution in MOPS;
- and optionally
- Glucose oxidase and HRP, preferably in solution in MOPS.
A kit comprising:
- Ascorbate oxidase and LeuDH in solution in MOPS;
- and
- Glucose oxidase and HRP in solution in MOPS.
Dependencies are inferred from the claim text.