Analysis method by electrophoresis
AssigneeACE BioAnalysis Inc.
InventorKiyohito SHIMURA
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Abstract
The present disclosure provides a method for analyzing proteins and polypeptides by electrophoresis using standards as internal standards. In one aspect, the present disclosure provides a method for measuring an analyte by electrophoresis, the method including the steps of preparing standards that do not contain tryptophan, subjecting a sample containing the analyte and the standards to electrophoresis simultaneously in the same separation field, detecting the analyte with an optical signal derived from tryptophan and detecting the standards with an optical signal of a wavelength different from that of the analyte, and measuring the analyte based on the optical signal. In one embodiment, the electrophoresis is capillary electrophoresis.
Claims19
1 A method for measuring an analyte, which is a protein and/or polypeptide, by electrophoresis, comprising the steps of:
- subjecting a sample containing the analyte and standards to electrophoresis simultaneously in the same separation space;
- detecting the analyte with an optical signal derived from tryptophan and detecting the standards with an optical signal of a different wavelength from the analyte;
- and measuring the analyte based on the optical signal.
An apparatus for measuring an analyte by electrophoresis, comprising:
- a flow path and electrodes for electrophoresis;
- a light source for illuminating a portion of the flow path with light;
- and
- a photodetector for receiving light from the flow path,
- wherein the photodetector is configured to detect light having a wavelength of 350 nm and light having a wavelength different from 350 nm.
A method for producing a standard substance for determining the molecular weight of an analyte, comprising the steps of:
- identifying a gene encoding a protein having a known molecular weight;
- identifying the base sequence encoding all tryptophan residues of the protein in the gene;
- obtaining a modified gene by replacing the portion encoding all tryptophan residues with a base sequence encoding amino acids other than tryptophan;
- obtaining a protein produced using the modified gene;
- and obtaining a conjugate formed by binding a labeling dye to the protein as the standard substance.
Dependencies are inferred from the claim text.