Compositions and methods for assay measurements
AssigneeMeso Scale Technologies, LLC.
InventorsAlexander K. TUCKER-SCHWARTZ, George SIGAL
Life of this patent
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Abstract
The invention relates to novel compositions comprising an electrochemiluminescence (ECL) co-reactant. In embodiments, the composition further comprises an ionic component, a surfactant, or combination thereof. In embodiments, the ECL co-reactant is triethanolamine (TEA), tert-butyldiethanolamine (tBDEA), methyldiethanolamine (MDEA), 3-[Bis-(2-hydroxy-ethyl)-amino]-propane-1-sulfonic acid (DEA-PS), or a combination thereof. Methods of using the compositions and kits comprising the compositions are also provided herein.
Claims166
A composition comprising:
- (a) triethanolamine (TEA);
- (b) an ionic component;
- and
- (c) an electrochemiluminescence (ECL)-labeled component,
- wherein the composition has a pH of about 7.0 to about 8.0, and wherein the composition is substantially free of an additional pH buffering component.
A composition consisting essentially of:
- (a) triethanolamine (TEA);
- (b) an ionic component;
- and (c) a surfactant, or
- (a) triethanolamine (TEA);
- (b) an ionic component;
- (c) a surfactant;
- and (d) an ECL-labeled component,
- wherein the composition has a pH of about 7.0 to about 8.0, and wherein the composition is substantially free of an additional pH buffering component.
A composition consisting of:
- (a) triethanolamine (TEA);
- (b) an ionic component;
- and (c) a surfactant, or
- (a) triethanolamine (TEA);
- (b) an ionic component;
- (c) a surfactant;
- and (d) an ECL-labeled component,
- wherein the composition has a pH of about 7.0 to about 8.0.
A composition comprising:
- (a) about 1000 mM to about 6500 mM of triethanolamine (TEA);
- and
- (b) about 500 mM to about 2000 mM of an ionic component;
- wherein the composition has a pH of about 7.0 to about 8.0.
A composition consisting essentially of:
- (a) about 1000 mM to about 6500 mM of triethanolamine (TEA);
- (b) about 500 mM to about 2000 mM of an ionic component;
- and (c) a surfactant, or
- (a) about 1000 mM to about 6500 mM of triethanolamine (TEA);
- (b) about 500 mM to about 2000 mM of an ionic component;
- (c) a surfactant;
- and (d) an ECL-labeled component,
- wherein the composition has a pH of about 7.0 to about 8.0.
A composition consisting of:
- (a) about 1000 mM to about 6500 mM of triethanolamine (TEA);
- (b) about 500 mM to about 2000 mM of an ionic component;
- and (c) a surfactant, or
- (a) about 1000 mM to about 6500 mM of triethanolamine (TEA);
- (b) about 500 mM to about 2000 mM of an ionic component;
- (c) a surfactant;
- and (d) an ECL-labeled component,
- wherein the composition has a pH of about 7.0 to about 8.0.
A composition comprising:
- (a) triethanolamine (TEA);
- (b) an ionic component;
- and
- (c) an alkyl ether-polyethylene glycol (PEG);
- wherein the composition has a pH of about 7.0 to about 8.0.
A composition consisting essentially of:
- (a) triethanolamine (TEA);
- (b) an ionic component;
- and (c) an alkyl ether-polyethylene glycol (PEG), or
- (a) triethanolamine (TEA);
- (b) an ionic component;
- (c) an alkyl ether-polyethylene glycol (PEG);
- and (d) an ECL-labeled component,
- wherein the composition has a pH of about 7.0 to about 8.0.
A composition consisting of:
- (a) triethanolamine (TEA);
- (b) an ionic component;
- and (c) an alkyl ether-polyethylene glycol (PEG), or
- (a) triethanolamine (TEA);
- (b) an ionic component;
- (c) an alkyl ether-polyethylene glycol (PEG);
- and (d) an ECL-labeled component,
- wherein the composition has a pH of about 7.0 to about 8.0.
A composition comprising:
- (a) triethanolamine (TEA);
- (b) an ionic component;
- and
- (c) optionally, one or both of an ECL-labeled component and a surfactant;
- wherein the composition has a pH of about 7.0 to about 8.0;
- and
- optionally, wherein the composition is substantially free of an additional pH buffering component.
A composition comprising:
- (a) an electrochemiluminescence (ECL) co-reactant selected from N-tert-butyldiethanolamine (tBDEA), methyldiethanolamine (MDEA), 3-[Bis-(2-hydroxy-ethyl)-amino]-propane-1-sulfonic acid (DEA-PS), and combination thereof;
- (b) an ionic component;
- and
- (c) a surfactant;
- wherein the composition has a pH of about 7.0 to about 8.0.
A composition consisting essentially of:
- (a) an electrochemiluminescence (ECL) co-reactant selected from N-tert-butyldiethanolamine (tBDEA), methyldiethanolamine (MDEA), 3-[Bis-(2-hydroxy-ethyl)-amino]-propane-1-sulfonic acid (DEA-PS), and combination thereof;
- (b) an ionic component;
- and (c) a surfactant, or
- (a) an electrochemiluminescence (ECL) co-reactant selected from N-tert-butyldiethanolamine (tBDEA), methyldiethanolamine (MDEA), 3-[Bis-(2-hydroxy-ethyl)-amino]-propane-1-sulfonic acid (DEA-PS), and combination thereof;
- (b) an ionic component;
- (c) a surfactant, and (d) an ECL-labeled component,
- wherein the composition has a pH of about 7.0 to about 8.0.
A composition consisting of:
- (a) an electrochemiluminescence (ECL) co-reactant selected from N-tert-butyldiethanolamine (tBDEA), methyldiethanolamine (MDEA), 3-[Bis-(2-hydroxy-ethyl)-amino]-propane-1-sulfonic acid (DEA-PS), and combination thereof;
- (b) an ionic component;
- (c) a surfactant;
- and (d) a pH buffering component, or
- (a) an electrochemiluminescence (ECL) co-reactant selected from N-tert-butyldiethanolamine (tBDEA), methyldiethanolamine (MDEA), 3-[Bis-(2-hydroxy-ethyl)-amino]-propane-1-sulfonic acid (DEA-PS), and combination thereof;
- (b) an ionic component;
- (c) a surfactant;
- (d) a pH buffering component, and (e) an ECL-labeled component,
- wherein the composition has a pH of about 7.0 to about 8.0.
A method for producing a composition, comprising combining:
- (a) triethanolamine (TEA);
- (b) an ionic component;
- (c) a surfactant;
- and
- (d) an ECL-labeled component,
- wherein the method does not comprise adding an additional pH buffering component.
An assay module comprising a TEA composition in dry form, wherein the TEA composition comprises TEA, an ionic component, and optionally a surfactant.
Dependencies are inferred from the claim text.