Back to results
US 2025/0354931 A1Published application
Spatial targeting of analytes
Assignee10x Genomics, Inc.
InventorsPreyas Shah, Eswar Prasad Ramachandran Iyer
Life of this patent
Estimating the term…
Abstract
Systems and methods are provided for targeting one or more features in a region of interest, and efficiently removing the features from an array of features. The systems and methods can remove one or more beads that contain analytes, intermediate agents, or a combination thereof, from the region of interest of a substrate by emitting light toward the beads on the substrate.
Claims21
A system comprising:
- a substrate comprising a feature array thereon, wherein the feature array comprises a plurality of features and analytes, intermediate agents, or a combination thereof that were previously captured by the plurality of features from a biological sample;
- a feature capture block including a capture surface, the capture surface configured to face the feature array on the substrate;
- a light generator configured to emit light;
- and
- a controller configured to activate the light generator to emit the light toward a portion of the capture surface of the feature capture block that corresponds to a region of interest on the feature array to expose the portion of the capture surface to the light such that a light-activated adhesive on the portion of the capture surface is activated to permit the capture surface to capture at least one feature from the feature array, the at least one feature comprising at least one element of the analytes, the intermediate agents, or the combination thereof.
2 more elements
(canceled)
A system comprising:
- a substrate comprising photo-sensitive bonds and a feature array attached to the substrate via the photo-sensitive bonds, wherein the feature array comprises a plurality of features and analytes, intermediate agents, or a combination thereof that were previously captured by the plurality of features from a biological sample;
- a light generator configured to emit light;
- a controller configured to activate the light generator to emit the light toward a portion a of the feature array to cleave the photo-sensitive bonds in a region of interest and permit for at least one feature within the region of interest of the feature array to be released from the substrate, the at least one feature comprising at least one element of the analytes, the intermediate agents, or the combination thereof;
- and
- a microscope configured to visualize the region of interest on the feature array.
2 more elements
Dependencies are inferred from the claim text.