Method for producing retinal tissue and retina-related cells
AssigneesSumitomo Chemical Company, Limited, Riken
InventorsTokushige NAKANO, Yoshiki SASAI (deceased), Chikafumi OZONE
Life of this patent
Estimating the term…
Abstract
The present invention provides a method for producing a retinal progenitor cell, including (1) a first step of subjecting pluripotent stem cells to floating culture in a serum-free medium to form an aggregate of pluripotent stem cells, and (2) a second step of subjecting the aggregate formed in step (1) to floating culture in a serum-free medium or serum-containing medium each being free of a substance acting on the Sonic hedgehog signal transduction pathway but containing a substance acting on the BMP signal transduction pathway, thereby obtaining an aggregate containing retinal progenitor cells.
Claims13
A method for producing a retinal progenitor cell, comprising
- (1) a first step of subjecting pluripotent stem cells to culture in a serum-free medium, and
- (2) a second step of subjecting the cells obtained in step (1) to culture in a serum-free medium or serum-containing medium each being free of a substance acting on the Sonic hedgehog signal transduction pathway and containing a substance acting on the BMP signal transduction pathway,
- thereby obtaining an aggregate containing retinal progenitor cells.
A method for producing a retinal tissue, comprising
- (1) a first step of subjecting pluripotent stem cells to culture in a serum-free medium,
- (2) a second step of subjecting the cells obtained in step (1) to culture in a serum-free medium or serum-containing medium each being free of a substance acting on the Sonic hedgehog signal transduction pathway and containing a substance acting on the BMP signal transduction pathway, thereby obtaining an aggregate containing retinal progenitor cells, and
- (3) a third step of subjecting the aggregate formed in step (2) to culture in a serum-free medium or serum-containing medium each being free of any of a substance acting on the Sonic hedgehog signal transduction pathway, a substance acting on the BMP signal transduction pathway and a substance acting on the Wnt signal pathway, thereby obtaining an aggregate containing retinal tissues and being substantially free of non-neural head ectoderm.
A method for producing a retinal layer-specific neural cell, comprising
- (1) a first step of subjecting pluripotent stem cells to culture in a serum-free medium,
- (2) a second step of subjecting the cells obtained in step (1) to culture in a serum-free medium or serum-containing medium each being free of a substance acting on the Sonic hedgehog signal transduction pathway and containing a substance acting on the BMP signal transduction pathway, thereby obtaining an aggregate containing retinal progenitor cells, and, and
- (3) a third step of subjecting the aggregate formed in step (2) to floating culture in a serum-free medium or serum-containing medium each being free of any of a substance acting on the Sonic hedgehog signal transduction pathway, a substance acting on the BMP signal transduction pathway and a substance acting on the Wnt signal pathway until the intended retinal layer-specific neural cells appear, thereby obtaining an aggregate containing retinal tissues containing the intended retinal layer-specific neural cells and being substantially free of non-neural head ectoderm.
Dependencies are inferred from the claim text.