Keratin Protein Extracts, Compositions, Methods and Uses Thereof
AssigneeStimLabs LLC
InventorsRachael Parker, Sarah Griffiths, Annelise Mowry Roy, Maryam Nuru, Brian Wicker
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Abstract
The present application relates to keratin protein extracts, compositions, methods and uses thereof. In some embodiments, the keratin protein extract is a beta-keratin extract. In some embodiments, the compositions are beta-keratin compositions. In some embodiments, the methods are methods of cleaning a keratin source material, methods of extracting beta-keratin, methods of preparing keratin compositions, and methods of using keratin protein extracts and compositions.
Claims144
A protein extract comprising beta-keratin monomers, wherein the extract has less than about 10.0 endotoxin units per mg of extract, such as less than about 0.80 endotoxin units per mg of extract.
A protein extract comprising beta-keratin monomers, wherein the extract has about 0.50 mM thiol groups when measured at about 0.1% weight extract per volume using Ellman's assay or above 0.20 mM thiol groups when measured at about 1% weight extract per volume using Ellman's assay.
A protein extract comprising beta-keratin monomers, wherein the extract when dehydrated is soluble or slightly soluble in a solvent having a pH from about 7 to about 8 at about 2.5% weight per volume.
A method of extracting beta-keratin monomers comprising mixing a beta-keratin source material with an extraction solution comprising urea at a concentration of at least about 1 M and thioglycolic acid.
A keratin composition comprising a beta-keratin extract, wherein the keratin composition has less than about 400 endotoxin units per composition.
A keratin composition comprising a beta-keratin network, wherein the keratin composition has less than about 400 endotoxin units per composition.
A keratin composition comprising a beta-keratin extract, wherein the keratin composition has less than about 400 endotoxin units per mg of the composition.
A keratin composition comprising a beta-keratin network, wherein the keratin composition has less than about 400 endotoxin units per mg of the composition.
A keratin composition comprising a beta-keratin extract, wherein the keratin composition has less than about 400 endotoxin units per cm2.
A keratin composition comprising a beta-keratin network, wherein the keratin composition has less than about 400 endotoxin units per cm2.
A keratin composition comprising a beta-keratin extract, wherein the keratin composition has less than about 400 endotoxin units per cm3.
A keratin composition comprising a beta-keratin network, wherein the keratin composition has less than about 400 endotoxin units per cm3.
A keratin composition comprising a beta-keratin network, wherein the keratin composition has a moisture vapor transmission rate that is at least about 1000 g/(m2*day) of material per day.
A keratin composition comprising a beta-keratin network, wherein the keratin composition has a thickness of greater than about 0.2 mm.
A keratin composition comprising a beta-keratin extract, wherein the keratin composition has less than about 400 endotoxin units per about 1 mL of the composition.
A keratin composition comprising a beta-keratin network, wherein the keratin composition has less than about 400 endotoxin units per about 1 mL of the composition.
A method of cleaning a beta-keratin source material comprising:
- a) combining a beta-keratin source material with a first solution comprising a first ionic detergent;
- b) soaking and/or agitating the beta-keratin source material in the first solution;
- c) rinsing the beta-keratin source material with water;
- d) soaking and/or agitating the beta-keratin source material in a second solution comprising a second ionic detergent;
- e) rinsing the beta-keratin source material with water;
- and
- f) optionally sterilizing the beta-keratin source material.
A method of cleaning a beta-keratin source material comprising:
- a) combining a beta-keratin source material with a first solution comprising at least about 0.1% v/v of a polyethylene glycol-based detergent in water;
- and
- b) soaking and/or agitating the beta-keratin source material in the first solution.
A method of cleaning a beta-keratin source material comprising:
- a) combining a beta-keratin source material with a first solution comprising a polyethylene glycol-based detergent;
- b) soaking and/or agitating the beta-keratin source material in the first solution;
- c) rinsing the beta-keratin source material with water and optionally drying the beta-keratin source material;
- d) soaking and/or agitating the beta-keratin source material in a second solution comprising a polyethylene glycol-based detergent;
- e) rinsing the beta-keratin source material with water;
- and
- f) optionally sterilizing the beta-keratin source material.
Dependencies are inferred from the claim text.