Back to results
US 2025/0352973 A1Published application
Row-independent oligonucleotide synthesis
AssigneeSierra BioSystems, Inc.
InventorsBruce ERICKSON, Marshall HENRY
Life of this patent
Estimating the term…
Abstract
Apparatuses and a method for plate-based oligonucleotide synthesis are disclosed. In one example, an apparatus used in oligonucleotide synthesis includes a synthesis adapter base to receive a commercially available synthesis plate. A keeper is used to apply pressure to the commercially available synthesis plate, and a sealing element is used to seal the commercially-available synthesis plate to the system adapter base. Other methods and apparatuses are disclosed.
Claims20
An apparatus comprising:
- one or more synthesis plates, each of the one or more synthesis plates having a plurality of rows, each row of the plurality of rows comprising a plurality of wells and each well of the plurality of wells comprising a waste tip;
- a synthesis adapter base;
- the system adapter base located beneath the one or more synthesis plates, wherein the synthesis adapter base is operably coupled to the synthesis plates by one or more fasteners;
- and
- a keeper located on top of the one or more synthesis plates, the keeper applying pressure to the one or more synthesis plates, thereby forming a seal around one or both of (i) each of the plurality of rows and (ii) each of the plurality of wells of the one or more synthesis plates.
2 more elements
An apparatus comprising:
- a chamber;
- one or more synthesis plates inside the chamber, each of the one or more synthesis plates having a plurality of rows, each row of the plurality of rows comprising a plurality of wells and each well of the plurality of wells comprising a waste tip;
- a synthesis adapter base having one or more adapter plates;
- the system adapter base located beneath the one or more synthesis plates;
- a synthesizer frame;
- wherein the synthesizer frame interfaces with the synthesis adapter base to provide structural support and alignment for the one or more synthesis plates.
- a keeper located on top of the one or more synthesis plates, the keeper applying pressure to the one or more synthesis plates, thereby forming a seal around one or both of (i) each of the plurality of rows and (ii) each of the plurality of wells of the one or more synthesis plates;
- one or more waste channels, the one or more waste channels dispensing reagents through the chamber to one or more outlets of the one or more adapter plates.
5 more elements
An apparatus comprising:
- a chamber comprising a lid;
- one or more synthesis plates inside the chamber, each of the one or more synthesis plates having a plurality of rows, each row of the plurality of rows comprising a plurality of wells and each well of the plurality of wells comprising a waste tip;
- a synthesis adapter base;
- the system adapter base located beneath the one or more adapter plates;
- and
- a keeper located on top of the one or more synthesis plates, the keeper applying pressure to the one or more synthesis plates, thereby forming a seal around one or both of (i) each of the plurality of rows and (ii) each of the plurality of wells of the one or more synthesis plates.
3 more elements
Dependencies are inferred from the claim text.