Highly accurate de novo polynucleotide synthesis
AssigneeTwist Bioscience Corporation
InventorsBill James PECK, Mary NOE, Stefan PITSCH, Patrick A. WEISS
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Abstract
Methods, systems, compositions, and devices for the manufacturing of high-quality building blocks, such as polynucleotides, are described herein. Processes described herein provide for efficient washing of residual reagents, solvents, or byproducts from previous synthetic steps to allow for the generation of polynucleotides with low error rates. Processes described herein also provide for reduction in deletion rates during chemical nucleic acid synthesis. Further, methods and devices described herein allow for the rapid construction and assembly of large libraries of highly accurate polynucleotides.
Claims21
-64. (canceled)
A method of polynucleotide synthesis comprising:
- providing a structure comprising a surface;
- coupling a nucleoside to a polynucleotide attached to the surface;
- depositing an oxidizing solution on the surface;
- depositing a wash solution on the surface, wherein the wash solution comprises water, a base, and a solvent.
A method for polynucleotide synthesis, the method comprising:
- providing a structure comprising a surface;
- coupling a nucleoside to a polynucleotide attached to the surface, wherein the nucleoside comprises a blocking group;
- depositing an oxidizing solution on the surface;
- depositing a wash solvent on the surface, wherein I2 or iodine salts have a greater solubility or increased rate of dissolution in the wash solvent compared to acetonitrile;
- and
- depositing a deblocking solution on the surface, wherein depositing the deblocking solution on the surface removes the blocking group.
Dependencies are inferred from the claim text.