Filter holders, filtration systems, methods of filtering a fluid, and methods of manufacturing a filtration system

A filter holder, comprising:

• a filter holder base having an aperture extending therethrough and a cavity formed at a first end thereof, wherein the filter holder base is shaped to receive and support a backing on a first surface thereof and to receive and support a filter membrane on a second surface thereof;
• a seal assembly configured to be coupled to the filter holder base and to sealingly engage a surface of the filter membrane when the filter membrane is on the second surface of the filter holder base;
• and
• a cover having an aperture formed therethrough and configured to be disposed over the seal assembly and over at least a portion of the filter holder base and secured to the filter holder base to substantially close the cavity.

A method of filtering a fluid, the method comprising:

• receiving a fluid into an aperture extending through a cover of a filter holder;
• filtering the fluid through a filter membrane disposed on a backing located within a cavity of a filter holder base;
• sealingly engaging a surface of the filter membrane via a seal assembly of the filter holder, wherein the cover is disposed over the seal assembly and at least a portion of the filter holder base, and wherein the cover is secured to the filter holder base to substantially close the cavity;
• and
• outputting the filtered fluid through an aperture extending through the filter holder base.

A method of manufacturing a filtration system, comprising:

• providing a filter holder base having an aperture extending therethrough and a cavity formed at a first end thereof;
• positioning a backing in the cavity of the filter holder base;
• positioning a filter membrane over the backing in the cavity;
• coupling a seal assembly to the filter holder base such that the seal assembly sealingly engages a surface of the filter membrane;
• and
• securing a cover over at least a portion of the filter holder base and the seal assembly to substantially close the cavity.