New multivalent hvt vector vaccine
AssigneeIntervet Inc.
InventorsMartijn Alexander LANGEREIS, Iwan VERSTEGEN, Paul VERMEIJ
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Abstract
The present invention regards a new recombinant HVT (rHVT) construct, useful as multivalent vector vaccine for poultry. The rHVT comprises 4 heterologous genes from poultry pathogens: the VP2 gene from IBDV, the F gene from NDV, and the gD and gI genes from ILTV. The VP2 and F genes are inserted in the Us genome region of the rHVT. The gD-gI genes are inserted in the UL genome region, between the UL54 and the LORF3 genes. The new rHVT-VP2-F-gD-gI proved to be genetically stable in vitro and in vivo, and expressed all inserted genes well. Also it was an effective vaccine against severe challenge infections with NDV, IBDV, and ILTV.
Claims15
A recombinant herpesvirus of turkeys (rHVT) comprising and expressing a first expression cassette which is inserted in the unique short (Us) region of the genome of the rHVT, and a second expression cassette which is inserted in the unique long (UL) region of the genome of the rHVT located between the UL54 and the LORF3 genes, the first expression cassette comprises an infectious bursal disease virus (IBDV) viral protein 2 (VP2) gene and a Newcastle disease virus (NDV) fusion (F) protein gene, and and the second expression cassette comprises a glycoprotein D and a glycoprotein I (gD and gI) gene of infectious laryngotracheitis virus (ILTV).
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