Accurate Method for Generating a Phase Diagram of a Polymer
AssigneeMax-Planck-Gesellschaft zur Forderung der Wissenschaften e.V.
InventorsAnatol W. Fritsch, Juan Manuel Iglesias Artola, Anthony A. Hyman
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Abstract
The present invention refers to a method for generating a binodal curve of a polymer in a system by determining accurate values of both concentration of dilute and condensed phases of a polymer, in particular, protein or polynucleic acid, under different condition, such as at different temperatures and salt concentrations. Furthermore, the present invention refers to an assay method for identify bioactive compound(s), comprising the inventive method for generating a binodal curve of a polymer in a system.
Claims17
A method for determining a concentration (ccon) of a polymer in a condensed phase and a concentration (cdil) of the polymer in a dilute phase in a system comprising:
- A) determining a total volume (Vtot) of the system and a total concentration (ctot) of the polymer;
- B) triggering phase separation of the polymer into a condensed phase and a dilute phase in said system;
- C) determining a volume fraction (Vcon/Vtot) of the condensed phase of the polymer in said system;
- D) determining a concentration (ccon) of the condensed phase of the polymer and a concentration (cdil) of the dilute phase of the polymer in said system by using a following linear form equation:
- V
- c
-
- o
-
- n
- V
- t
-
- o
-
- t
- =
- 1
- c
- con
- -
- c
- dil
- ·
- c
- tot
- -
- c
- dil
- c
- con
- -
- c
- dil
- ,
- E) changing at least one condition of said system and repeating the steps A) to D).
An assay method for identifying bioactive compound(s), comprising:
- A) determining a total volume (Vtot) of the system and a total concentration (ctot) of a polymer in the system;
- A′) adding a predetermined amount of test compounds into said system;
- B) triggering phase separation of the polymer into a condensed phase and a dilute phase;
- C) determining a volume fraction (Vcon/Vtot) of the condensed phase of the polymer in said system;
- D) determining a concentration (ccon) of the condensed phase of the polymer and a concentration (cdil) of the dilute phase of the polymer in said system by using a following linear form equation:
- V
- c
-
- o
-
- n
- V
- t
-
- o
-
- t
- =
- 1
- c
- con
- -
- c
- dil
- ·
- c
- tot
- -
- c
- dil
- c
- con
- -
- c
- dil
- ,
- D′) comparing the measured condensed volume (Vcon), the measured condensed concentration (ccon), and/or the measured volume fraction (Vcon/Vtot) of said polymer in the system with a condensed volume (Vref), a condensed concentration (cref), and/or a volume fraction (Vref/Vtot) of the polymer in the presence of a reference molecule in a control system;
- F) identifying the bioactive compounds from the test compounds;
- and a device comprises a plurality of systems.
A device for determining a binodal curve of a polymer in a system comprising:
- i) means for measuring a total volume (Vtot) of the system;
- ii) means for measuring a volume fraction (Vcon/Vtot) of a condensed phase of the polymer in said system;
- iii) means for calculating a concentration (ccon) of the condensed phase of the polymer and a concentration (cdil) of the dilute phase of the polymer in said system by using a following linear form equation:
- V
- c
-
- o
-
- n
- V
- t
-
- o
-
- t
- =
- 1
- c
- con
- -
- c
- dil
- ·
- c
- tot
- -
- c
- dil
- c
- con
- -
- c
- dil
- .
Dependencies are inferred from the claim text.