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US 2025/0340910 A1Published application
Fatty acid production in escherichia coli
OwnerAbel Juan BAERGA ORTIZ, Melissa C. ORTIZ-ROSARIOinventors
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Abstract
The disclosure provides methods for increasing saturated, monounsaturated, and poly unsaturated fatty acid production using the PUFA gene cluster plasmid and the gene regulator pfaR.
Claims12
An in vitro method of producing fatty acids, wherein an E. coli containing pfaA, pfaB, pfaC, pfaD and/or pfaE genes (pEPAΔ1, 3, 4, 9) overexpresses gene regulator pfaR.
The method of clam 1, wherein the E. coli is incubated at 15° C.
A pfaRso plasmid, wherein the gene sequence of pfaRso gene has greater than 90% homology to SEQ ID NO. 1.
Dependencies are inferred from the claim text.