Identification of cervical biomarkers
AssigneeAkna Health Inc.
InventorSascha DREWLO
Life of this patent
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Abstract
Provided herein are methods and kits for identifying a pregnancy-associated risk or condition in a subject. In embodiments, the methods include detecting levels of at least one biomarker in a substantially cell-free cervical fluid sample obtained from the subject. In embodiments, the methods include detecting levels of biomarkers in a cervical fluid sample including extravillous trophoblasts (EVT) obtained from the subject.
Claims46
A method of identifying a pregnancy-associated risk or condition in a subject, the method comprising:
- obtaining a cervical fluid sample from a subject, the cervical fluid sample comprising no greater than 1 cell per 1 milliliter (mL) volume;
- and
- detecting an elevated level or a decreased level of at least one biomarker in the cervical fluid sample relative to a standard control, thereby identifying the pregnancy associated risk or condition.
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A method of identifying one or more pregnancy-associated risks or conditions in a subject, the method comprising:
- a) obtaining a biological sample from the cervix of the subject, wherein the biological sample comprises extravillous trophoblast (EVT) cells;
- b) performing single-cell time-of-flight mass spectrometry (CyTOF-MS) on the biological sample to generate an output;
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- c) determining an elevated level or a decreased level of at least one biomarker in the biological sample relative to a standard control based on the output.
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A method of identifying a pregnancy-associated risk or condition in a subject, the method comprising:
- a) obtaining a biological sample from the cervix of the subject, wherein the biological sample comprises extravillous trophoblast (EVT) cells and biological material derived from the cervix of the subject, the biological material comprising at least 90% weight by volume (w/v) of the biological sample;
- b) performing single-cell time-of-flight mass spectrometry (CyTOF-MS) on the biological sample to generate an output;
- and
- c) determining an elevated level or a decreased level of at least one biomarker in the biological sample relative to a standard control based on the output, thereby identifying the one or more pregnancy-associated risks or conditions.
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A kit for obtaining a biological sample from a subject, comprising:
- a collection device;
- a collection container comprising a stabilizing solution.
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Dependencies are inferred from the claim text.