Detecting methylcytosine using a modified base opposite to the methylcytosine
AssigneeIllumina, Inc.
InventorsColin Brown, Sarah Shultzaberger, Eric Brustad
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Abstract
Examples provided herein are related to detecting methylcytosine using a modified base opposite to the methylcytosine. A methylcytosine in a first polynucleotide including a plurality of cytosines may be detected, using a method that includes hybridizing the first polynucleotide to a second polynucleotide. The second polynucleotide may include a modified base opposite to the methylcytosine. The methylcytosine may be detected using the modified base. For example, the modified base may include a fluorophore. The methylcytosine may be detected using fluorescence from the fluorophore responsive to excitation light.
Claims36
A method for detecting a methylcytosine in a first polynucleotide comprising a plurality of cytosines, the method comprising:
- hybridizing the first polynucleotide to a second polynucleotide,
- wherein the second polynucleotide comprises a modified base opposite to the methylcytosine, wherein the modified base comprises a solvatochromatic nucleoside or a modified base comprising a target capable of coupling to a fluorophore;
- and
- detecting the methylcytosine using the modified base.
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Dependencies are inferred from the claim text.