Methods of modifying methylcytosine or derivative thereof using a nucleophilic molecule, and methods of using the same to detect the methylcytosine or derivative thereof in a polynucleotide

Abstract

Disclosed herein are methods of modifying 5-methylcytosine (5-mC), 5-hydroxymethylcytosine (5-hmC), or 5-formlcytosine (5-fC) in a polynucleotide. The method may include oxidizing the 5-mC, 5-hmC, or 5-fC to 5-carboxylcytosine (5-caC); activating the 5-carboxyl group of the 5-caC; and reacting the activated 5-carboxyl group with a nucleophilic molecule to form a product. In some examples, the product may be used to detect the 5-mC, 5-hmC, or 5-fC in the polynucleotide.

Dependencies are inferred from the claim text.