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US 2025/0320548 A1Published application
Extraction-free pathogen testing methods
AssigneesTransformative Biotech, LLC, The Regents of the University of Colorado
InventorsRobert E. Blomquist, Shi-Long Lu, Brian Harry, Jose P. Zevallos, Xin Yao
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Abstract
The invention provides compositions and methods allowing for rapid, accurate, robust, and low-cost diagnosis of infectious diseases via extraction-free, direct PCR techniques.
Claims21
A method for extraction-free analysis of nucleic acid, the method comprising the steps of:
- mixing a biological sample in a buffer composition comprising nuclease-free water, an antifungal, an antibiotic, a ribonuclease inhibitor, and a reducing agent;
- directly amplifying said nucleic acid in said buffer with primers specific to a target nucleic acid without prior extraction of said nucleic acid;
- and
- analyzing amplicons produced in said amplifying step to detect presence of a pathogen.
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Dependencies are inferred from the claim text.