Improved methods and cells for increasing enzyme activity and production of insect pheromones
AssigneeFmc Agricul Tural Solutions A/S
InventorsIrina Borodina, Carina Holkenbrink, Kanchana Rueksomtawin Kildegaard, Karolis Petkevicius, Leonie Wenning
Life of this patent
Estimating the term…
Abstract
Herein are provided methods for increasing enzymatic activity of desaturases and fatty acyl reductases by co-expressing NAD(P)H cytochrome b5 reductases (EC 1.6.2.2, Ncb5or), as well as to methods for production of compounds comprised in pheromones, in particular moth pheromones, such as saturated and desaturated fatty alcohols, and saturated and desaturated fatty alcohol acetates and fatty acids, and derivatives thereof, in a cell.
Claims16
A cell expressing:
- i) a first enzyme or group of enzymes capable of converting a fatty acyl-CoA to a desaturated fatty alcohol, a saturated fatty alcohol, a desaturated fatty alcohol acetate, a desaturated fatty acyl-CoA, or a combination thereof, and
- ii) a heterologous NAD(P)H cytochrome b5 oxidoreductase (Ncb5or);
- whereby the cell is capable of producing the compound with a higher titer and/or purity compared to a cell expressing the first group of enzymes but no heterologous Ncb5or when cultivated in the same conditions,
- preferably wherein said cell is a yeast cell, such as an oleaginous yeast cell, or a plant cell.
A system of nucleic acid constructs comprising nucleic acids encoding an Ncb5or and:
- a. a desaturase capable of introducing at least one double bond in a fatty acyl-CoA;
- and/or
- b. a fatty acyl-CoA reductase capable of converting at least part of a desaturated fatty acyl-CoA to a desaturated fatty alcohol.
Dependencies are inferred from the claim text.