Chromosomal integrating cassette allowing inducible gene expression for production of compounds via fermentation

A transcription control cassette composed for the expression of at least one gene of interest, characterized in that the transcription control cassette comprises the following parts i) through vi),

• i) a first DNA sequence comprising a promoter DNA sequence, being flanked by a first Lac operator DNA sequence and a second Lac operator DNA sequence;
• ii) a second DNA sequence encoding for a repressor to bind the Lac operators of i);
• iii) a third DNA sequence comprising a second nucleotide promotor sequence to drive expression of ii), wherein the second nucleotide promotor is the same nucleotide promotor as is comprised by part i) or a different one;
• iv) a fourth DNA sequence comprising a transcription terminator sequence which is terminating the transcription of part vi;
• v) a fifth DNA sequence comprising a sequence for a selection marker;
• vi) a sixth DNA sequence comprising at least one fragment from the at least one gene of interest of suitable length which is ensuring the homologous recombination with the chromosomal sequence of the cell into which the transcription control cassette is to be transformed, wherein the fragment from the at least one gene of interest is comprising the first nucleotides of the respective gene of interest;
• wherein the DNA sequences of any two or more of parts i) through vi)
• are connected directly to each other within the complete sequence of the transcription control cassette
• or
• are separated from each other within the complete sequence of the transcription control cassette by DNA sequences of DNA restriction cleavage sites.