Compositions and methods of nucleic acid-targeting nucleic acids
AssigneeCaribou Biosciences, Inc.
InventorsAndrew Paul May, Rachel E. Haurwitz, Jennifer A. Doudna, James M. Berger, Matthew Merrill Carter, Paul Daniel Donohoue
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Abstract
This disclosure provides for compositions and methods for the use of nucleic acid-targeting nucleic acids and complexes thereof. Genome engineering can refer to altering the genome by deleting, inserting, mutating, or substituting specific nucleic acid sequences. The altering can be gene or location specific. Genome engineering can use nucleases to cut a nucleic acid thereby generating a site for the alteration. Engineering of non-genomic nucleic acid is also contemplated.
Claims8
1-55. (canceled)
A method for sequencing a target nucleic acid, the method comprising:
- a. contacting a first target site in a nucleic acid with a first complex comprising a first CRISPR Cas9 protein and a first guide RNA and a second target site in the nucleic acid with a second complex comprising a second CRISPR Cas9 protein and a second guide RNA, whereby the first Cas9 protein and the first guide RNA bind and cleave the first target site, and the second Cas9 protein and the second guide RNA bind and cleave the second target site, thereby producing a cleaved target nucleic acid;
- b. ligating adapters to the ends of the cleaved target nucleic acid, and
- c. sequencing the cleaved target nucleic acid.
Dependencies are inferred from the claim text.