Three-dimensional structure, method of modeling bronchiolitis obliterans syndrome, and associated methods

Abstract

The present disclosure relates to a three-dimensional structure including an inverted co-culture having an interior layer comprising a plurality of cells of a first cell type; an opposing exterior layer comprising a plurality cells of a second cell type; and a basement membrane matrix positioned between the interior layer and the exterior layer, wherein the three-dimensional structure has a diameter, and wherein said diameter is capable of being altered by an alloimmune reaction that disrupts the plurality of cells of the second cell type and leads to contraction of the plurality of cells of the second cell type. Also disclosed is a method of modeling Bronchiolitis Obliterans Syndrome, a method for assessing presence of or risk of developing a physiological condition, a method of identifying one or more biomarkers of a disease, and a method of a method of making a three-dimensional structure.

Dependencies are inferred from the claim text.