Fusion protein containing anti-tigit antibody and tgf-ßr, and pharmaceutical composition and use thereof

A fusion protein, comprising:

• a first protein functional region targeting TIGIT, and
• a second protein functional region having TGF-β binding activity;
• wherein,
• the number of the first protein functional region is 1, and the number of the second protein functional region is 2;
• the first protein functional region is an anti-TIGIT antibody or an antigen-binding fragment thereof, and the second protein functional region is a TGF-β receptor, an extracellular region thereof, or a truncated fragment of a TGF-β receptor extracellular region with TGF-β receptor functions;
• a heavy chain of the anti-TIGIT antibody comprises an amino acid sequence set forth in SEQ ID NO:
• 27 or SEQ ID NO:
• 31, and a light chain thereof comprises an amino acid sequence set forth in SEQ ID NO:
• 29;
• the second protein functional region comprises an amino acid sequence set forth in SEQ ID NO:
• 33;
• the second protein functional region is linked to the C-terminus of the heavy chain of the anti-TIGIT antibody via a linker fragment;
• preferably, the linker fragment comprises an amino acid sequence set forth in SEQ ID NO:
• 44.