Immunoassay for detecting biologically active proteins

An immunoassay for detecting a biologically active antigen, particularly a hormone, a protein or a pharmaceutical substance, in a biological sample from an individual, provided with:

• a sample application area for applying the biological sample from the individual, wherein the biological sample is preferably urine, whole blood, saliva, milk or serum;
• a capture area, wherein the capture area is provided with an immobilized first antibody that is directed against the biologically active antigen, in particular against the hormone, the protein, the peptide or the pharmaceutical substance;
• a conjugate area, wherein the conjugate area is provided with a second antibody directed against the biologically active antigen, particularly against the hormone, protein, peptide or pharmaceutical substance;
• characterized in that
• the first antibody and the second antibody are a recombinant antibody obtained from a diatom or unicellular plant,
• wherein the immunoassay is provided with at least one further antibody and/or one auxiliary protein,
• wherein the auxiliary protein, as a blocker, saturates free surfaces of a reaction vessel or a membrane,
• wherein the other antibody and the auxiliary protein are obtained from the diatom and/or a Viridiplantae and/or the unicellular plant, and
• wherein the recombinant antibody is provided in a purity of at least 90%, and
• wherein the immunoassay is vegan.