Bio-based production of succinic acid using vibrio natriegens
AssigneeGeneral Biological Corporation
InventorsDavid Kendall Sozanski, Kyle Phillip Mohler, Abhinav Godavarthi, Timothy J. Hanly
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Abstract
This disclosure provides methods and genetically engineered strains of Vibrio natriegens, specifically developed for the bio-based production of succinate. Capitalizing on the rapid growth kinetics and highly efficient carbon metabolism of V. natriegens, this disclosure provides an environmentally friendly, scalable, and cost-effective alternative to traditional petrochemical methods for succinate production.
Claims36
A method of producing succinic acid, the method comprising:
- a. culturing a plurality of non-naturally occurring Vibrio natriegens cells comprising one or more genetic disruptions, wherein the one or more genetic disruptions increase the production of succinate as compared to the production of succinate from a V. natriegens cell that does not comprise the one or more genetic disruptions;
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- b. wherein under anaerobic conditions, the plurality of non-naturally occurring V. natriegens cells produce succinic acid from a glucose substrate at a rate of at least 0.48 gSucc gCDW−1 h−1.
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A method of producing succinic acid, comprising culturing a population of genetically modified Vibrio natriegens cells in a two-phase cultivation for a period of greater than 24 hours, wherein the population of genetically modified V. natriegens cells comprise a deletion of each of the lldH, dldH, alD, and pflB genes.
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An engineered Vibrio natriegens cell comprising one or more genetic disruptions, wherein the one or more genetic disruptions increase the production of succinate as compared to the production of succinate from a V. natriegens cell that does not comprise the one or more genetic disruptions, wherein the V. natriegens cell produces succinic acid from a glucose substrate at a rate of at least 0.48 gSucc gCDW−1 h−1.
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Dependencies are inferred from the claim text.