Control of phenotype in plants
AssigneeGreenvenus, LLC
InventorsSekhar BODDUPALLI, Andrey BOUKHAROV, Rio STAMLER, Zhongsen LI, Arianne TREMBLAY, Stephen SCHAUER, Shiv B. TIWARI, John SALMERON
Life of this patent
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Abstract
The invention provides a compositions and methods for controlling phenotypic traits in plants. Genes of interest are placed under the control of a gene switch to allow inducible control or expression of a gene of interest “on-demand” by treatment of the plant with a chemical ligand.
Claims64
One or more vectors or polynucleotides comprising:
- (a) a polynucleotide encoding an ecdysone receptor-based (EcR-based) gene switch or an ecdysone receptor-based (EcR-based) gene switch comprising a VGEvy ligand binding domain;
- (b) a nucleic acid which controls a trait or traits in a plant;
- and,
- (c) a regulatory element operably connected 5′ of b), wherein said EcR-based gene switch induces expression of b) in the presence of a chemical ligand.
One or more vectors or polynucleotides comprising:
- (a) a polynucleotide encoding an ecdysone receptor-based (EcR-based) gene switch or an ecdysone receptor-based (EcR-based) gene switch comprising a VGEvy ligand binding domain;
- (b) a nucleic acid which controls flowering in plants;
- and,
- (c) a regulatory element 5′ of b), wherein said EcR-based gene switch induces expression of b) in the presence of a chemical ligand.
One or more vectors or polynucleotides comprising:
- a) a polynucleotide encoding an ecdysone receptor-based (EcR-based) gene switch or an ecdysone receptor-based (EcR-based) gene switch comprising a VGEvy ligand binding domain;
- b) an immutans variegation mutant (IM) nucleic acid;
- and,
- c) a regulatory element 5′ of b), wherein said EcR-based gene switch induces expression of b) in the presence of a chemical ligand.
One or more vectors or polynucleotides comprising:
- a) a polynucleotide encoding an ecdysone receptor-based (EcR-based) gene switch or an ecdysone receptor-based (EcR-based) gene switch comprising a VGEvy ligand binding domain;
- b) a nucleic acid encoding a protein or an RNA that inhibits a plant pathogen;
- and,
- c) a regulatory element 5′ of b), wherein said EcR-based gene switch induces expression of b) in the presence of a chemical ligand.
One or more vectors or polynucleotides comprising nucleic acids encoding:
- (a) an ecdysone receptor-based (EcR-based) gene switch;
- (b) a Bacillus amyloliquefaciens Barstar protein;
- (c) a Bacillus amyloliquefaciens barnase ribonuclease;
- and,
- (d) a regulatory element 5′ of (b), wherein said EcR-based gene switch induces expression of b) in the presence of a chemical ligand.
The one or more vectors or polynucleotides in any one of the preceding claims, wherein said EcR-based gene switch further comprises a heterologous (non-Cf) DNA-binding domain and a heterologous (non-Cf) transcriptional transactivation domain.
The one or more vectors or polynucleotides of any of the preceding claims, wherein said EcR-based gene switch comprises a transactivation domain comprising one or more of a Group H nuclear receptor member transactivation domain, steroid/thyroid hormone nuclear receptor transactivation domain, synthetic or chimeric transactivation domain, polyglutamine transactivation domain, basic or acidic amino acid transactivation domain, a VP16 transactivation domain, a GAL4 transactivation domain, an NF-κB transactivation domain, a BP64 transactivation domain, a B42 acidic transactivation domain (B42AD), a p53 transactivation domain, a p65 transactivation domain (p65AD), an EDLL acidic transactivation domain from AP2/ERF, a glutamine-rich transactivation domain, an ARFs or CONSTANS protein transactivation domain, a proline-rich domain transactivation domain from bZIP or an analog, combination, or modification thereof.
The one or more vectors or polynucleotides of any one of the preceding claims, wherein said EcR-based gene switch comprises the polypeptide sequence of SEQ ID NO:1.
The one or more vectors or polynucleotides in any one of the preceding claims, wherein said chemical ligand is a non-steroidal chemical ligand.
The one or more vectors or polynucleotides of any one of the preceding claims further comprising a nucleic acid sequence encoding a second gene switch and a second gene of interest wherein said second gene of interest is operatively linked to a second regulatory element operatively linked 5′ of said second gene of interest wherein said second gene switch binds said second regulatory element in the presence of a second chemical ligand.
The one or more vectors or polynucleotides of 35 wherein said second gene switch comprises any of an ecdysone receptor (EcR) based gene switch, a rapamycin-based gene switch, a prokaryotic tetracycline repressor-based gene switch, a lactose repressor-operator-based gene switch, a cumate-based gene switch, or a biotin-based gene switch.
The one or more vectors or polynucleotides of any of the preceding claims, wherein said nucleic acid further encodes a polypeptide comprising a repression domain comprising any of an AUX/IAA protein with an LxLxL domain, or an ERF-associated amphiphilic repression (EAR) domain.
A plant comprising the one or more vectors or polynucleotides of any one of the preceding claims.
A method of generating increased efficiency or effectiveness in methods of commercial seed production, comprising introduction of one or more vectors or polynucleotides of the invention into said plants.
One or more vectors or polynucleotides comprising:
- (a) a polynucleotide encoding an ecdysone receptor-based (EcR-based) gene switch or an ecdysone receptor-based (EcR-based) gene switch comprising a VGEvy ligand binding domain;
- (b) a nucleic acid which downregulates trait or traits in a plant;
- and,
- (c) a regulatory element operably connected to a repressor element, wherein said EcR-based gene switch induces expression of said repressor element in the presence of a chemical ligand and said repressor element represses (b).
Dependencies are inferred from the claim text.