Replicon Compositions and Methods of Using Same for the Treatment of Diseases

A method for the treatment or prevention of a bacterial, viral, parasitical or fungal infection in a subject, said method comprising administering to the subject a composition comprising:

• at least two replicable RNA molecules, each comprising a first open-reading frame (ORF) encoding at least one peptide or protein comprising an antigen or epitope suitable to induce an immune response against the bacterium, virus, parasite or fungus, respectively;
• wherein the at least one peptide or protein encoded by one of the replicable RNA molecules is different from the at least one peptide or protein encoded by the other replicable RNA molecule,
• optionally wherein at least one of the replicable RNA molecules further comprises a second ORF encoding an RNA-dependent RNA polymerase (replicase) capable of replicating in cis or in trans the replicable RNA molecules.