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US 2025/0277731 A1Published application
Particles characterization in flow cytometry
AssigneeBeckman Coulter, Inc.
InventorIhor BEREZHNYY
Life of this patent
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Abstract
A method of characterizing particles in flow cytometry includes determining a first pulse width value of a particle using a first technique. The method includes determining a second pulse width value of the particle using a second technique. The method further includes comparing the first and second pulse width values, and characterizing the particle as a concatenated particle when a difference between the first and second pulse width values exceeds a threshold.
Claims11
A system for characterizing particles, the system comprising:
- a light emitting unit generating one or more excitation light beams;
- a fluidic system streaming the particles through the one or more excitation light beams at an interrogation zone;
- optical elements directing scattered light from the interrogation zone;
- one or more detectors measuring characteristics of the scattered light;
- and
- a processing circuitry having non-transitory computer readable storage media storing instructions which, when executed by the processing circuity, cause the processing circuitry to:
- determine first pulse width values of the particles using a first technique;
- determine second pulse width values of the particles using a second technique;
- compare the first and second pulse width values;
- and
- characterize the particles as concatenated particles when a difference between the first and second pulse width values exceeds a threshold.
8 more elements
Dependencies are inferred from the claim text.